Molecular changes associated with adaptation of equine influenza H3N8 virus in embryonated chicken eggs

Awlad Wadair Ali Said, Michiko Kodani, Tatsufumi Usui, Yoshikazu Fujimoto, Toshihiro Ito, Tsuyoshi Yamaguchi

研究成果: ジャーナルへの寄稿コメント/討論査読

4 被引用数 (Scopus)

抄録

Embryonated chicken eggs (ECEs) are routinely used to isolate equine influenza virus. Propagation of the virus in ECEs results in selection of variants. In the present study, we determined nucleotide sequences of entire coding regions of parent A/equine/ Tottori/1/07 (H3N8) and its derivatives that have different passage histories in ECE. After 12 passages, nucleotide sequence analysis predicted 3 amino acid substitutions in hemagglutinin (HA; 2 in HA1 and 1 in HA2). The two amino acid substitutions in HA1 were located in the vicinity of the cell receptor-binding site. Three other amino acid substitutions were predicted in internal proteins, 1 in the M1, 1 in the NP and 1 in the PA. This is the first report showing mutations in the internal protein genes of equine influenza virus associated with adaptation to ECE.

本文言語英語
ページ(範囲)545-548
ページ数4
ジャーナルJournal of Veterinary Medical Science
73
4
DOI
出版ステータス出版済み - 4月 2011

!!!All Science Journal Classification (ASJC) codes

  • 獣医学(全般)

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