Molecular characteristics of colorectal neuroendocrine carcinoma; Similarities with adenocarcinoma rather than neuroendocrine tumor

Nobuyoshi Takizawa, Yoshihiro Ohishi, Minako Hirahashi, Shunsuke Takahashi, Kazuhiko Nakamura, Masao Tanaka, Eiji Oki, Ryoichi Takayanagi, Yoshinao Oda

研究成果: ジャーナルへの寄稿記事

27 引用 (Scopus)

抄録

To further clarify the molecular features of colorectal neuroendocrine carcinomas (NECs), we immunohistochemically examined tumor samples from 25 NECs, including 9 small cell NECs (SCNECs) and 16 large cell NECs (LCNECs), 20 neuroendocrine tumors (NETs), and 21 poorly differentiated adenocarcinomas (PDCs) for the expression of several biomarkers (p53, β-catenin, Bcl-2, Rb, p16, p21, cyclin D1, and cyclin E) and used sequencing analysis to identify gene alterations of TP53, APC, CTNNB1, KRAS, and BRAF. The frequencies of aberrant p53 expression (88%), β-catenin nuclear expression (48%), and high expression of cyclin E (84%) were significantly higher in NECs than in NETs (0%, 5%, and 5%, P <.01, respectively). The immunohistochemical results of NECs and PDCs were similar. TP53, APC, KRAS, and BRAF gene mutations were variously detected in NECs and PDCs but not in any NETs. The frequencies of decreased expression of Rb (56%) and high expression of p16 (56%) and Bcl-2 (64%) were significantly higher in NECs than in PDCs (5%, 19%, and 5%, P <.05, respectively) or NETs (10%, 5%, and 5%, P <.01, respectively). Such immunohistochemical characteristics of NECs were more evident in SCNECs than in large cell NECs (P <.01). In conclusion, the molecular features of colorectal NECs are similar to those of adenocarcinomas and not to those of NETs. Decreased expression of Rb and high expression of p16 and Bcl-2 are characteristics of NECs, suggesting that Rb-p16 pathway disruption may contribute to the promotion of proliferative activity in colorectal NECs. SCNECs may be a prototype of NECs.

元の言語英語
ページ(範囲)1890-1900
ページ数11
ジャーナルHuman Pathology
46
発行部数12
DOI
出版物ステータス出版済み - 12 1 2015

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Neuroendocrine Carcinoma
Neuroendocrine Tumors
Colorectal Neoplasms
Adenocarcinoma
Large Cell Carcinoma
Catenins
Cyclin E
Small Cell Carcinoma
Cyclin D1
p53 Genes

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine

これを引用

Molecular characteristics of colorectal neuroendocrine carcinoma; Similarities with adenocarcinoma rather than neuroendocrine tumor. / Takizawa, Nobuyoshi; Ohishi, Yoshihiro; Hirahashi, Minako; Takahashi, Shunsuke; Nakamura, Kazuhiko; Tanaka, Masao; Oki, Eiji; Takayanagi, Ryoichi; Oda, Yoshinao.

:: Human Pathology, 巻 46, 番号 12, 01.12.2015, p. 1890-1900.

研究成果: ジャーナルへの寄稿記事

Takizawa, Nobuyoshi ; Ohishi, Yoshihiro ; Hirahashi, Minako ; Takahashi, Shunsuke ; Nakamura, Kazuhiko ; Tanaka, Masao ; Oki, Eiji ; Takayanagi, Ryoichi ; Oda, Yoshinao. / Molecular characteristics of colorectal neuroendocrine carcinoma; Similarities with adenocarcinoma rather than neuroendocrine tumor. :: Human Pathology. 2015 ; 巻 46, 番号 12. pp. 1890-1900.
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abstract = "To further clarify the molecular features of colorectal neuroendocrine carcinomas (NECs), we immunohistochemically examined tumor samples from 25 NECs, including 9 small cell NECs (SCNECs) and 16 large cell NECs (LCNECs), 20 neuroendocrine tumors (NETs), and 21 poorly differentiated adenocarcinomas (PDCs) for the expression of several biomarkers (p53, β-catenin, Bcl-2, Rb, p16, p21, cyclin D1, and cyclin E) and used sequencing analysis to identify gene alterations of TP53, APC, CTNNB1, KRAS, and BRAF. The frequencies of aberrant p53 expression (88{\%}), β-catenin nuclear expression (48{\%}), and high expression of cyclin E (84{\%}) were significantly higher in NECs than in NETs (0{\%}, 5{\%}, and 5{\%}, P <.01, respectively). The immunohistochemical results of NECs and PDCs were similar. TP53, APC, KRAS, and BRAF gene mutations were variously detected in NECs and PDCs but not in any NETs. The frequencies of decreased expression of Rb (56{\%}) and high expression of p16 (56{\%}) and Bcl-2 (64{\%}) were significantly higher in NECs than in PDCs (5{\%}, 19{\%}, and 5{\%}, P <.05, respectively) or NETs (10{\%}, 5{\%}, and 5{\%}, P <.01, respectively). Such immunohistochemical characteristics of NECs were more evident in SCNECs than in large cell NECs (P <.01). In conclusion, the molecular features of colorectal NECs are similar to those of adenocarcinomas and not to those of NETs. Decreased expression of Rb and high expression of p16 and Bcl-2 are characteristics of NECs, suggesting that Rb-p16 pathway disruption may contribute to the promotion of proliferative activity in colorectal NECs. SCNECs may be a prototype of NECs.",
author = "Nobuyoshi Takizawa and Yoshihiro Ohishi and Minako Hirahashi and Shunsuke Takahashi and Kazuhiko Nakamura and Masao Tanaka and Eiji Oki and Ryoichi Takayanagi and Yoshinao Oda",
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AU - Takizawa, Nobuyoshi

AU - Ohishi, Yoshihiro

AU - Hirahashi, Minako

AU - Takahashi, Shunsuke

AU - Nakamura, Kazuhiko

AU - Tanaka, Masao

AU - Oki, Eiji

AU - Takayanagi, Ryoichi

AU - Oda, Yoshinao

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AB - To further clarify the molecular features of colorectal neuroendocrine carcinomas (NECs), we immunohistochemically examined tumor samples from 25 NECs, including 9 small cell NECs (SCNECs) and 16 large cell NECs (LCNECs), 20 neuroendocrine tumors (NETs), and 21 poorly differentiated adenocarcinomas (PDCs) for the expression of several biomarkers (p53, β-catenin, Bcl-2, Rb, p16, p21, cyclin D1, and cyclin E) and used sequencing analysis to identify gene alterations of TP53, APC, CTNNB1, KRAS, and BRAF. The frequencies of aberrant p53 expression (88%), β-catenin nuclear expression (48%), and high expression of cyclin E (84%) were significantly higher in NECs than in NETs (0%, 5%, and 5%, P <.01, respectively). The immunohistochemical results of NECs and PDCs were similar. TP53, APC, KRAS, and BRAF gene mutations were variously detected in NECs and PDCs but not in any NETs. The frequencies of decreased expression of Rb (56%) and high expression of p16 (56%) and Bcl-2 (64%) were significantly higher in NECs than in PDCs (5%, 19%, and 5%, P <.05, respectively) or NETs (10%, 5%, and 5%, P <.01, respectively). Such immunohistochemical characteristics of NECs were more evident in SCNECs than in large cell NECs (P <.01). In conclusion, the molecular features of colorectal NECs are similar to those of adenocarcinomas and not to those of NETs. Decreased expression of Rb and high expression of p16 and Bcl-2 are characteristics of NECs, suggesting that Rb-p16 pathway disruption may contribute to the promotion of proliferative activity in colorectal NECs. SCNECs may be a prototype of NECs.

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