Molecular characterization and expression analysis of a phosphoserine aminotransferase involving l-serine synthesis from silkworm, Bombyx mori

Mohammad R. Haque, Aiko Hirowatari, Ayumi Koyanagi, Takashi Ichinose, Maiko Abiru, Shinya Mohri, Shigeki Furuya, Kohji Yamamoto

研究成果: Contribution to journalArticle査読

1 被引用数 (Scopus)

抄録

In this study, we identified and characterized a phosphoserine aminotransferase (bmPSAT) from Bombyx mori (B. mori) that is responsible for l-serine biosynthesis. A complementary DNA that encodes bmPSAT was cloned by reverse transcriptase polymerase reaction and sequenced. The presumed amino acid sequence revealed 47–87% identity with known PSATs from insects, humans, plants, and bacteria. Through phylogenetic analysis, we found that bmPSAT is evolutionary related to insect PSATs. Recombinant bmPSAT was produced in Escherichia coli by using a cold-shock promotor and purified to homogeneity. This enzyme utilizes phosphohydroxypyruvate and glutamate for transamination. bmPSAT messenger RNA (mRNA) was expressed at higher levels in several tissues of standard strain silkworm including the silk gland, whereas a sericin-deficient silkworm strain exhibited a diminished expression of bmPSAT mRNA in the silk gland. These findings indicate that bmPSAT may play an important role in synthesizing and supplying l-serine in the larva of B. mori.

本文言語英語
論文番号e21553
ジャーナルArchives of insect biochemistry and physiology
101
2
DOI
出版ステータス出版済み - 6 2019

All Science Journal Classification (ASJC) codes

  • 生理学
  • 生化学
  • 昆虫科学

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