Molecular characterization and expression analysis of a phosphoserine aminotransferase involving l-serine synthesis from silkworm, Bombyx mori

Mohammad R. Haque, Aiko Hirowatari, Ayumi Koyanagi, Takashi Ichinose, Maiko Abiru, Shinya Mohri, Shigeki Furuya, Kohji Yamamoto

研究成果: ジャーナルへの寄稿記事

抄録

In this study, we identified and characterized a phosphoserine aminotransferase (bmPSAT) from Bombyx mori (B. mori) that is responsible for l-serine biosynthesis. A complementary DNA that encodes bmPSAT was cloned by reverse transcriptase polymerase reaction and sequenced. The presumed amino acid sequence revealed 47–87% identity with known PSATs from insects, humans, plants, and bacteria. Through phylogenetic analysis, we found that bmPSAT is evolutionary related to insect PSATs. Recombinant bmPSAT was produced in Escherichia coli by using a cold-shock promotor and purified to homogeneity. This enzyme utilizes phosphohydroxypyruvate and glutamate for transamination. bmPSAT messenger RNA (mRNA) was expressed at higher levels in several tissues of standard strain silkworm including the silk gland, whereas a sericin-deficient silkworm strain exhibited a diminished expression of bmPSAT mRNA in the silk gland. These findings indicate that bmPSAT may play an important role in synthesizing and supplying l-serine in the larva of B. mori.

元の言語英語
記事番号e21553
ジャーナルArchives of insect biochemistry and physiology
101
発行部数2
DOI
出版物ステータス出版済み - 6 1 2019

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phosphoserine aminotransferase
silk glands
Bombyx
Silk
transaminases
Bombyx mori
messenger RNA
silkworms
serine
Serine
Sericins
transamination
Messenger RNA
insects
complementary DNA
synthesis
RNA-directed DNA polymerase
RNA-Directed DNA Polymerase
Biosynthesis
cold stress

All Science Journal Classification (ASJC) codes

  • Physiology
  • Biochemistry
  • Insect Science

これを引用

Molecular characterization and expression analysis of a phosphoserine aminotransferase involving l-serine synthesis from silkworm, Bombyx mori. / Haque, Mohammad R.; Hirowatari, Aiko; Koyanagi, Ayumi; Ichinose, Takashi; Abiru, Maiko; Mohri, Shinya; Furuya, Shigeki; Yamamoto, Kohji.

:: Archives of insect biochemistry and physiology, 巻 101, 番号 2, e21553, 01.06.2019.

研究成果: ジャーナルへの寄稿記事

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abstract = "In this study, we identified and characterized a phosphoserine aminotransferase (bmPSAT) from Bombyx mori (B. mori) that is responsible for l-serine biosynthesis. A complementary DNA that encodes bmPSAT was cloned by reverse transcriptase polymerase reaction and sequenced. The presumed amino acid sequence revealed 47–87{\%} identity with known PSATs from insects, humans, plants, and bacteria. Through phylogenetic analysis, we found that bmPSAT is evolutionary related to insect PSATs. Recombinant bmPSAT was produced in Escherichia coli by using a cold-shock promotor and purified to homogeneity. This enzyme utilizes phosphohydroxypyruvate and glutamate for transamination. bmPSAT messenger RNA (mRNA) was expressed at higher levels in several tissues of standard strain silkworm including the silk gland, whereas a sericin-deficient silkworm strain exhibited a diminished expression of bmPSAT mRNA in the silk gland. These findings indicate that bmPSAT may play an important role in synthesizing and supplying l-serine in the larva of B. mori.",
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AU - Koyanagi, Ayumi

AU - Ichinose, Takashi

AU - Abiru, Maiko

AU - Mohri, Shinya

AU - Furuya, Shigeki

AU - Yamamoto, Kohji

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