Molecular characterization and isolation of cytochrome P450 genes from the filamentous fungus Aspergillus oryzae

K. H.M. Nazmul Hussain Nazir, Hirofumi Ichinose, Hiroyuki Wariishi

研究成果: Contribution to journalArticle査読

32 被引用数 (Scopus)

抄録

We explored the molecular diversity of cytochrome P450 genes in the filamentous fungus Aspergillus oryzae using bioinformatic and experimental approaches. Based on bioinformatic annotation, we found 155 putative genes of cytochromes P450 in the whole genome sequence; however, 13 of 155 appeared to be pseudogenes due to sequence deletions and/or inframe stop codon(s). There are 87 families of A. oryzae cytochromes P450 (AoCYPs), indicating considerable phylogenetic diversity. To characterize A. oryzae AoCYPs, we attempted to isolate cDNAs using RT-PCR and determined their transcriptional capabilities. To date, we have confirmed gene expression of 133 AoCYPs and cloned 121 AoCYPs as full-length cDNAs encoding a mature open reading frame. Using experimentally deduced sequences and intron-exon organization, we analyzed AoCYPs phylogenetically. We also identified intronic consensus sequences in AoCYPs genes. The experimentally validated exonic and intronic sequences will be a powerful advantage in identification and characterization of novel P450s from various ascomycetous fungi.

本文言語英語
ページ(範囲)395-408
ページ数14
ジャーナルArchives of Microbiology
192
5
DOI
出版ステータス出版済み - 5 2010

All Science Journal Classification (ASJC) codes

  • 微生物学
  • 生化学
  • 分子生物学
  • 遺伝学

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