Molecular characterization and isolation of cytochrome P450 genes from the filamentous fungus Aspergillus oryzae

K. H.M. Nazmul Hussain Nazir, Hirofumi Ichinose, Hiroyuki Wariishi

研究成果: ジャーナルへの寄稿記事

31 引用 (Scopus)

抄録

We explored the molecular diversity of cytochrome P450 genes in the filamentous fungus Aspergillus oryzae using bioinformatic and experimental approaches. Based on bioinformatic annotation, we found 155 putative genes of cytochromes P450 in the whole genome sequence; however, 13 of 155 appeared to be pseudogenes due to sequence deletions and/or inframe stop codon(s). There are 87 families of A. oryzae cytochromes P450 (AoCYPs), indicating considerable phylogenetic diversity. To characterize A. oryzae AoCYPs, we attempted to isolate cDNAs using RT-PCR and determined their transcriptional capabilities. To date, we have confirmed gene expression of 133 AoCYPs and cloned 121 AoCYPs as full-length cDNAs encoding a mature open reading frame. Using experimentally deduced sequences and intron-exon organization, we analyzed AoCYPs phylogenetically. We also identified intronic consensus sequences in AoCYPs genes. The experimentally validated exonic and intronic sequences will be a powerful advantage in identification and characterization of novel P450s from various ascomycetous fungi.

元の言語英語
ページ(範囲)395-408
ページ数14
ジャーナルArchives of Microbiology
192
発行部数5
DOI
出版物ステータス出版済み - 5 1 2010

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Aspergillus oryzae
Aspergillus
Fungi
Cytochrome P-450 Enzyme System
Genes
Bioinformatics
Computational Biology
Complementary DNA
Pseudogenes
Terminator Codon
Sequence Deletion
Consensus Sequence
Gene expression
Introns
Open Reading Frames
Exons
Genome
Gene Expression
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Biochemistry
  • Molecular Biology
  • Genetics

これを引用

Molecular characterization and isolation of cytochrome P450 genes from the filamentous fungus Aspergillus oryzae. / Nazmul Hussain Nazir, K. H.M.; Ichinose, Hirofumi; Wariishi, Hiroyuki.

:: Archives of Microbiology, 巻 192, 番号 5, 01.05.2010, p. 395-408.

研究成果: ジャーナルへの寄稿記事

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