Molecular characterization of a heat shock cognate 70-4 promoter from the silkworm, Bombyx mori

Man Lee, Takahiro Kusakabe, Yutaka Kawaguchi, Chisa Yasunaga-Aoki, Si Kab Nho, Yumiko Nakajima, Katsumi Koga

研究成果: ジャーナルへの寄稿記事

11 引用 (Scopus)

抄録

A cDNA stretch encoding one of the silkworm HSC70 genes with its upstream sequence has been identified, based on partial cDNA sequences registered in a Bombyx mori EST database. The deduced amino acid sequence with 649 residues was 89 and 96% identical to those from Drosophila melanogaster hsc4 and Manduca sexta Hsc70-4, respectively. The expression analysis by the reverse transcription polymerase chain reaction demonstrated that the mRNA transcription occurred in all tissues examined and was not stimulated by heat shock. These results suggested that the current structure corresponds to the silkworm heat shock cognate 70-4 gene and named BmHSC70-4. Cloning and sequencing of the genomic 5'-flanking, putative promoter region of BmHSC70-4 revealed the presence of several canonical transcription elements such as the GATA box, CCAAT motif and HSE (heat shock element), although without a TATA box element. The deletion analysis of the 5' flanking region of the BmHSC70-4 gene, that fused to the firefly luciferase coding sequence as a reporter, indicated that the longest construct (-1,639/+26) showed the highest promoter activity, and the constructs -111 to -59 and -1,110 to -941 contained the basal transcription enhancer and the repressor elements, respectively.

元の言語英語
ページ(範囲)33-39
ページ数7
ジャーナルJournal of Insect Biotechnology and Sericology
72
発行部数1
出版物ステータス出版済み - 2 2003

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Bombyx
Transcription
Bombyx mori
silkworms
heat stress
Shock
transcription (genetics)
Hot Temperature
promoter regions
Genes
Complementary DNA
Firefly Luciferases
Manduca
TATA box
TATA Box
genes
5' Flanking Region
Expressed Sequence Tags
Manduca sexta
luciferase

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Business, Management and Accounting(all)
  • Agricultural and Biological Sciences(all)
  • Industrial and Manufacturing Engineering
  • Insect Science

これを引用

Molecular characterization of a heat shock cognate 70-4 promoter from the silkworm, Bombyx mori. / Lee, Man; Kusakabe, Takahiro; Kawaguchi, Yutaka; Yasunaga-Aoki, Chisa; Nho, Si Kab; Nakajima, Yumiko; Koga, Katsumi.

:: Journal of Insect Biotechnology and Sericology, 巻 72, 番号 1, 02.2003, p. 33-39.

研究成果: ジャーナルへの寄稿記事

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abstract = "A cDNA stretch encoding one of the silkworm HSC70 genes with its upstream sequence has been identified, based on partial cDNA sequences registered in a Bombyx mori EST database. The deduced amino acid sequence with 649 residues was 89 and 96{\%} identical to those from Drosophila melanogaster hsc4 and Manduca sexta Hsc70-4, respectively. The expression analysis by the reverse transcription polymerase chain reaction demonstrated that the mRNA transcription occurred in all tissues examined and was not stimulated by heat shock. These results suggested that the current structure corresponds to the silkworm heat shock cognate 70-4 gene and named BmHSC70-4. Cloning and sequencing of the genomic 5'-flanking, putative promoter region of BmHSC70-4 revealed the presence of several canonical transcription elements such as the GATA box, CCAAT motif and HSE (heat shock element), although without a TATA box element. The deletion analysis of the 5' flanking region of the BmHSC70-4 gene, that fused to the firefly luciferase coding sequence as a reporter, indicated that the longest construct (-1,639/+26) showed the highest promoter activity, and the constructs -111 to -59 and -1,110 to -941 contained the basal transcription enhancer and the repressor elements, respectively.",
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AU - Yasunaga-Aoki, Chisa

AU - Nho, Si Kab

AU - Nakajima, Yumiko

AU - Koga, Katsumi

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