Molecular characterization of fungal populations on the tongue dorsum of institutionalized elderly adults

H. Li, T. Takeshita, M. Furuta, M. Tomioka, Y. Shibata, Y. Shimazaki, K. Makimura, Y. Yamashita

研究成果: ジャーナルへの寄稿記事

6 引用 (Scopus)

抄録

Objectives: To characterize the global composition of oral fungal populations in frail elderly adults and to investigate the relationship with their health status. Materials and methods: We investigated the fungal populations on the tongue dorsum in 291 institutionalized elderly adults by molecular PCR-based techniques using internal transcribed spacer regions of nuclear ribosomal DNA. Results: Quantitative PCR analysis showed that fungi were present on the tongue dorsum of 128 subjects at ≥104CFU per sample, and 35 of them exceeded 105CFU per sample. Length heterogeneity-PCR analysis and nucleotide sequence determinations showed that Candida albicans was most frequently detected in those subjects with fungi at ≥104CFU per sample (105 subjects), followed by Candida dubliniensis (78), Malassezia restricta (57), and Candida tropicalis (45). Statistical analysis revealed that those subjects with ≥105CFU of fungi other than C. albicans per sample had an increased risk of fever (≥7 febrile days per 12months) compared with subjects with <105CFU per sample, after adjustment for other fever-associated confounding factors. Conclusions: These data demonstrate that the oral cavity of the elderly is inhabited by a diverse array of fungi not limited to typical Candida species and they suggest that the diversity in distribution is associated with health status.

元の言語英語
ページ(範囲)771-777
ページ数7
ジャーナルOral Diseases
18
発行部数8
DOI
出版物ステータス出版済み - 11 1 2012

Fingerprint

Tongue
Fungi
Fever
Candida albicans
Candida
Polymerase Chain Reaction
Population
Health Status
Malassezia
Candida tropicalis
Frail Elderly
Ribosomal DNA
Sequence Analysis
Mouth

All Science Journal Classification (ASJC) codes

  • Otorhinolaryngology
  • Dentistry(all)

これを引用

Molecular characterization of fungal populations on the tongue dorsum of institutionalized elderly adults. / Li, H.; Takeshita, T.; Furuta, M.; Tomioka, M.; Shibata, Y.; Shimazaki, Y.; Makimura, K.; Yamashita, Y.

:: Oral Diseases, 巻 18, 番号 8, 01.11.2012, p. 771-777.

研究成果: ジャーナルへの寄稿記事

@article{793d0bdc987b4f1c870a3b6d0ea837cc,
title = "Molecular characterization of fungal populations on the tongue dorsum of institutionalized elderly adults",
abstract = "Objectives: To characterize the global composition of oral fungal populations in frail elderly adults and to investigate the relationship with their health status. Materials and methods: We investigated the fungal populations on the tongue dorsum in 291 institutionalized elderly adults by molecular PCR-based techniques using internal transcribed spacer regions of nuclear ribosomal DNA. Results: Quantitative PCR analysis showed that fungi were present on the tongue dorsum of 128 subjects at ≥104CFU per sample, and 35 of them exceeded 105CFU per sample. Length heterogeneity-PCR analysis and nucleotide sequence determinations showed that Candida albicans was most frequently detected in those subjects with fungi at ≥104CFU per sample (105 subjects), followed by Candida dubliniensis (78), Malassezia restricta (57), and Candida tropicalis (45). Statistical analysis revealed that those subjects with ≥105CFU of fungi other than C. albicans per sample had an increased risk of fever (≥7 febrile days per 12months) compared with subjects with <105CFU per sample, after adjustment for other fever-associated confounding factors. Conclusions: These data demonstrate that the oral cavity of the elderly is inhabited by a diverse array of fungi not limited to typical Candida species and they suggest that the diversity in distribution is associated with health status.",
author = "H. Li and T. Takeshita and M. Furuta and M. Tomioka and Y. Shibata and Y. Shimazaki and K. Makimura and Y. Yamashita",
year = "2012",
month = "11",
day = "1",
doi = "10.1111/j.1601-0825.2012.01944.x",
language = "English",
volume = "18",
pages = "771--777",
journal = "Oral Diseases",
issn = "1354-523X",
publisher = "Wiley-Blackwell",
number = "8",

}

TY - JOUR

T1 - Molecular characterization of fungal populations on the tongue dorsum of institutionalized elderly adults

AU - Li, H.

AU - Takeshita, T.

AU - Furuta, M.

AU - Tomioka, M.

AU - Shibata, Y.

AU - Shimazaki, Y.

AU - Makimura, K.

AU - Yamashita, Y.

PY - 2012/11/1

Y1 - 2012/11/1

N2 - Objectives: To characterize the global composition of oral fungal populations in frail elderly adults and to investigate the relationship with their health status. Materials and methods: We investigated the fungal populations on the tongue dorsum in 291 institutionalized elderly adults by molecular PCR-based techniques using internal transcribed spacer regions of nuclear ribosomal DNA. Results: Quantitative PCR analysis showed that fungi were present on the tongue dorsum of 128 subjects at ≥104CFU per sample, and 35 of them exceeded 105CFU per sample. Length heterogeneity-PCR analysis and nucleotide sequence determinations showed that Candida albicans was most frequently detected in those subjects with fungi at ≥104CFU per sample (105 subjects), followed by Candida dubliniensis (78), Malassezia restricta (57), and Candida tropicalis (45). Statistical analysis revealed that those subjects with ≥105CFU of fungi other than C. albicans per sample had an increased risk of fever (≥7 febrile days per 12months) compared with subjects with <105CFU per sample, after adjustment for other fever-associated confounding factors. Conclusions: These data demonstrate that the oral cavity of the elderly is inhabited by a diverse array of fungi not limited to typical Candida species and they suggest that the diversity in distribution is associated with health status.

AB - Objectives: To characterize the global composition of oral fungal populations in frail elderly adults and to investigate the relationship with their health status. Materials and methods: We investigated the fungal populations on the tongue dorsum in 291 institutionalized elderly adults by molecular PCR-based techniques using internal transcribed spacer regions of nuclear ribosomal DNA. Results: Quantitative PCR analysis showed that fungi were present on the tongue dorsum of 128 subjects at ≥104CFU per sample, and 35 of them exceeded 105CFU per sample. Length heterogeneity-PCR analysis and nucleotide sequence determinations showed that Candida albicans was most frequently detected in those subjects with fungi at ≥104CFU per sample (105 subjects), followed by Candida dubliniensis (78), Malassezia restricta (57), and Candida tropicalis (45). Statistical analysis revealed that those subjects with ≥105CFU of fungi other than C. albicans per sample had an increased risk of fever (≥7 febrile days per 12months) compared with subjects with <105CFU per sample, after adjustment for other fever-associated confounding factors. Conclusions: These data demonstrate that the oral cavity of the elderly is inhabited by a diverse array of fungi not limited to typical Candida species and they suggest that the diversity in distribution is associated with health status.

UR - http://www.scopus.com/inward/record.url?scp=84866864275&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84866864275&partnerID=8YFLogxK

U2 - 10.1111/j.1601-0825.2012.01944.x

DO - 10.1111/j.1601-0825.2012.01944.x

M3 - Article

C2 - 22642872

AN - SCOPUS:84866864275

VL - 18

SP - 771

EP - 777

JO - Oral Diseases

JF - Oral Diseases

SN - 1354-523X

IS - 8

ER -