Molecular characterization of fungal populations on the tongue dorsum of institutionalized elderly adults

H. Li, T. Takeshita, M. Furuta, M. Tomioka, Y. Shibata, Y. Shimazaki, K. Makimura, Y. Yamashita

研究成果: Contribution to journalArticle査読

7 被引用数 (Scopus)


Objectives: To characterize the global composition of oral fungal populations in frail elderly adults and to investigate the relationship with their health status. Materials and methods: We investigated the fungal populations on the tongue dorsum in 291 institutionalized elderly adults by molecular PCR-based techniques using internal transcribed spacer regions of nuclear ribosomal DNA. Results: Quantitative PCR analysis showed that fungi were present on the tongue dorsum of 128 subjects at ≥104CFU per sample, and 35 of them exceeded 105CFU per sample. Length heterogeneity-PCR analysis and nucleotide sequence determinations showed that Candida albicans was most frequently detected in those subjects with fungi at ≥104CFU per sample (105 subjects), followed by Candida dubliniensis (78), Malassezia restricta (57), and Candida tropicalis (45). Statistical analysis revealed that those subjects with ≥105CFU of fungi other than C. albicans per sample had an increased risk of fever (≥7 febrile days per 12months) compared with subjects with <105CFU per sample, after adjustment for other fever-associated confounding factors. Conclusions: These data demonstrate that the oral cavity of the elderly is inhabited by a diverse array of fungi not limited to typical Candida species and they suggest that the diversity in distribution is associated with health status.

ジャーナルOral Diseases
出版ステータス出版済み - 11 2012

All Science Journal Classification (ASJC) codes

  • 耳鼻咽喉科学
  • 歯科学(全般)


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