Molecular cloning and characterization of the translationally controlled tumor protein gene in Bombyx mori

Jae Man Lee, Takahiro Kusakabe, Yutaka Kawaguchi, Yoshitaka Miyagawa, Masateru Takahashi, Hiroaki Mon, Si Kab Nho, Katsumi Koga

研究成果: ジャーナルへの寄稿記事

21 引用 (Scopus)

抄録

Translationally controlled tumor protein (Tctp/p23) is known to be synthesized preferentially in cells during the early growth phase of tumors, but is also expressed in normal cells. To elucidate its molecular basis of the expression and physiological significance, a cDNA encoding for the Bombyx mori Tctp (BmTctp) was deduced by editing the partial cDNA sequences registered in a Bombyx EST database. RT-PCR analyses indicated that the BmTCTP mRNA was transcribed in all larval organs examined and was present constantly during the cell cycle of BmN4 cells. A genomic clone of 4255 nucloetide residues produced by inverse PCR contained the 5′-flanking region, two introns and three exons of the BmTCTP gene. Sequence analysis of the 5′-flanking region indicated that a putative promoter region contains several canonical transcription elements such as GATA box, CCAAT motif, MEF2, E4BP4.01 and AP-1, but lacks a TATA box element. Luciferase reporter assay of the deletion constructs of the 5′-flanking region revealed that the -676 to +66 region enhanced the promoter activity the most markedly. In addition to this, there were at least two enhancer-like elements and several repressor elements.

元の言語英語
ページ(範囲)35-43
ページ数9
ジャーナルComparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
139
発行部数1
DOI
出版物ステータス出版済み - 9 1 2004

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Bombyx
5' Flanking Region
Cloning
Molecular Cloning
Genes
Genetic Promoter Regions
Complementary DNA
Polymerase Chain Reaction
TATA Box
Expressed Sequence Tags
Transcription Factor AP-1
Transcription
Luciferases
Introns
Sequence Analysis
Tumors
Exons
Assays
Cell Cycle
Clone Cells

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Physiology
  • Molecular Biology

これを引用

Molecular cloning and characterization of the translationally controlled tumor protein gene in Bombyx mori. / Lee, Jae Man; Kusakabe, Takahiro; Kawaguchi, Yutaka; Miyagawa, Yoshitaka; Takahashi, Masateru; Mon, Hiroaki; Nho, Si Kab; Koga, Katsumi.

:: Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology, 巻 139, 番号 1, 01.09.2004, p. 35-43.

研究成果: ジャーナルへの寄稿記事

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abstract = "Translationally controlled tumor protein (Tctp/p23) is known to be synthesized preferentially in cells during the early growth phase of tumors, but is also expressed in normal cells. To elucidate its molecular basis of the expression and physiological significance, a cDNA encoding for the Bombyx mori Tctp (BmTctp) was deduced by editing the partial cDNA sequences registered in a Bombyx EST database. RT-PCR analyses indicated that the BmTCTP mRNA was transcribed in all larval organs examined and was present constantly during the cell cycle of BmN4 cells. A genomic clone of 4255 nucloetide residues produced by inverse PCR contained the 5′-flanking region, two introns and three exons of the BmTCTP gene. Sequence analysis of the 5′-flanking region indicated that a putative promoter region contains several canonical transcription elements such as GATA box, CCAAT motif, MEF2, E4BP4.01 and AP-1, but lacks a TATA box element. Luciferase reporter assay of the deletion constructs of the 5′-flanking region revealed that the -676 to +66 region enhanced the promoter activity the most markedly. In addition to this, there were at least two enhancer-like elements and several repressor elements.",
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AU - Mon, Hiroaki

AU - Nho, Si Kab

AU - Koga, Katsumi

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