Molecular cloning and characterization of type III polyketide synthase from Plumbago zeylanica

Seiichi Sakamoto, Yui Morita, Gorawit Yusakul, Waraporn Putalun, Hiroyuki Tanaka, Satoshi Morimoto

研究成果: ジャーナルへの寄稿学術誌査読

2 被引用数 (Scopus)

抄録

Two cDNAs encoding type Ш polyketide synthase (PKS1) and chalcone synthase (CHS, PKS2), were cloned from fresh leaves of Plumbago zeylanica L. (P. zeylanica). Their heterologous expression revealed that PKS1 catalyzed the formation of five α-pyrones from three to six acetate units by accepting acetyl-CoA and malonyl-CoA. In contrast, PKS2 catalyzed the formation of naringenin and bisnoryangonin by accepting p-coumaroyl-CoA and malonyl-CoA. Naringenin is thought to be involved in the biosynthesis of various bioactive flavonoids. PKS2 can be used to molecular breeding to enhance the production of these useful secondary metabolites via its overexpression. (Figure presented.).

本文言語英語
ページ(範囲)478-490
ページ数13
ジャーナルJournal of Asian Natural Products Research
23
5
DOI
出版ステータス出版済み - 2021

!!!All Science Journal Classification (ASJC) codes

  • 分析化学
  • 分子医療
  • 薬理学
  • 薬科学
  • 創薬
  • 補完代替医療
  • 有機化学

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