TY - JOUR
T1 - Molecular cloning and determination of the nucleotide sequence of a gene encoding salt-tolerant glutaminase from Micrococcus luteus K-3
AU - Wakayama, Mamoru
AU - Nagano, Yoshio
AU - Renu, Nandakumar
AU - Kawamura, Tatsuro
AU - Sakai, Kenji
AU - Moriguchi, Mitsuaki
PY - 1996/1/1
Y1 - 1996/1/1
N2 - The gene encoding the salt-tolerant glutaminase I from marine Micrococcus luteus K-3 (M. luteus K-3) was cloned in Escherichia coli (E. coli) JM109. Clones were screened by hybridization with degenerate oligonucleotide probes designed using the known N-terminal amino acid sequence of glutaminase I from M. luteus K-3. A 2.4-kb HincII fragment from a 8-kb primary cloned DNA fragment was subcloned and sequenced. This fragment had an open reading frame of 1,368 nucleotides encoding 456 amino acids. The molecular weight of the deduced amino acid sequence of glutaminase I was determined to be 48,247. Comparison of the amino acid sequence of glutaminase I with those of kidney, brain, liver, and Caenorhabditis elegans glutaminases revealed high degrees of homology in several local regions.
AB - The gene encoding the salt-tolerant glutaminase I from marine Micrococcus luteus K-3 (M. luteus K-3) was cloned in Escherichia coli (E. coli) JM109. Clones were screened by hybridization with degenerate oligonucleotide probes designed using the known N-terminal amino acid sequence of glutaminase I from M. luteus K-3. A 2.4-kb HincII fragment from a 8-kb primary cloned DNA fragment was subcloned and sequenced. This fragment had an open reading frame of 1,368 nucleotides encoding 456 amino acids. The molecular weight of the deduced amino acid sequence of glutaminase I was determined to be 48,247. Comparison of the amino acid sequence of glutaminase I with those of kidney, brain, liver, and Caenorhabditis elegans glutaminases revealed high degrees of homology in several local regions.
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U2 - 10.1016/S0922-338X(97)81259-7
DO - 10.1016/S0922-338X(97)81259-7
M3 - Article
AN - SCOPUS:0030497434
VL - 82
SP - 592
EP - 597
JO - Journal of Bioscience and Bioengineering
JF - Journal of Bioscience and Bioengineering
SN - 1389-1723
IS - 6
ER -