Molecular cloning and expression of human hepatocyte growth factor

Toshikazu Nakamura, Tsutomu Nishizawa, Mitchio Hagiya, Tatsuya Seki, Manabu Shimonishi, Atsushi Sugimura, Kosuke Tashiro, Shin Shimizu

研究成果: ジャーナルへの寄稿記事

1882 引用 (Scopus)

抄録

Hepatocyte growth factor (HGF) is the most potent mitogen for mature parenchyma! hepatocytes in primary culture, and seems to be a hepatotrophic factor that acts as a trigger for liver regeneration after partial hepatectomy and liver injury. The partial purification and characterization of HGF have been reported1-4. We have demonstrated that pure HGF from rat platelets is a new growth factor5, 6 effective at concentrations as low as 1 ng mP-1.The effects of HGF and epidermal growth factor (EGF) are additive. The activity of HGF is not species-specific, although it does not stimulate growth in Swiss 3T3 fibroblasts5. HGF has a relative molecular mass (Mr) of 82,000 and is a heterodimer composed of a large a-subunit of Mr 69,000 and a small β -subunit of Mr34,000 (ref. 6). Here we report the amino-acid sequence of human HGF determined by complementary DNA cloning and the expression of biologically active human HGF from COS-1 cells transfected with cloned cDNA. The nucleotide sequence of the human HGF cDNA reveals that both α- andβ-chains are contained in a single open reading frame coding for a pre-pro precursor protein of 728 amino acids.

元の言語英語
ページ(範囲)440-443
ページ数4
ジャーナルNature
342
発行部数6248
DOI
出版物ステータス出版済み - 1 1 1989

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Hepatocyte Growth Factor
Molecular Cloning
Complementary DNA
Liver Regeneration
Protein Precursors
COS Cells
Hepatectomy
Growth
Mitogens
Epidermal Growth Factor
Open Reading Frames
human HGF protein
Organism Cloning
Hepatocytes
Amino Acid Sequence
Blood Platelets
Amino Acids
Liver
Wounds and Injuries

All Science Journal Classification (ASJC) codes

  • General

これを引用

Nakamura, T., Nishizawa, T., Hagiya, M., Seki, T., Shimonishi, M., Sugimura, A., ... Shimizu, S. (1989). Molecular cloning and expression of human hepatocyte growth factor. Nature, 342(6248), 440-443. https://doi.org/10.1038/342440a0

Molecular cloning and expression of human hepatocyte growth factor. / Nakamura, Toshikazu; Nishizawa, Tsutomu; Hagiya, Mitchio; Seki, Tatsuya; Shimonishi, Manabu; Sugimura, Atsushi; Tashiro, Kosuke; Shimizu, Shin.

:: Nature, 巻 342, 番号 6248, 01.01.1989, p. 440-443.

研究成果: ジャーナルへの寄稿記事

Nakamura, T, Nishizawa, T, Hagiya, M, Seki, T, Shimonishi, M, Sugimura, A, Tashiro, K & Shimizu, S 1989, 'Molecular cloning and expression of human hepatocyte growth factor', Nature, 巻. 342, 番号 6248, pp. 440-443. https://doi.org/10.1038/342440a0
Nakamura T, Nishizawa T, Hagiya M, Seki T, Shimonishi M, Sugimura A その他. Molecular cloning and expression of human hepatocyte growth factor. Nature. 1989 1 1;342(6248):440-443. https://doi.org/10.1038/342440a0
Nakamura, Toshikazu ; Nishizawa, Tsutomu ; Hagiya, Mitchio ; Seki, Tatsuya ; Shimonishi, Manabu ; Sugimura, Atsushi ; Tashiro, Kosuke ; Shimizu, Shin. / Molecular cloning and expression of human hepatocyte growth factor. :: Nature. 1989 ; 巻 342, 番号 6248. pp. 440-443.
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