Molecular cloning and sequence analysis of cDNA coding for rat liver hemoprotein H-450

Satoru Ishihara, Ken-Ichirou Morohashi, Hiroyuki Sadano, Shun-Ichiro Kawabata, Osamu Gotoh, Tsuneo Omura

研究成果: ジャーナルへの寄稿記事

19 引用 (Scopus)

抄録

cDNA clones coding for hemoprotein H-450 were isolated from a rat liver cDNA library using anti-H-450 antibody. The molecular weight calculated from the deduced amino acid sequence comprising 547 amino acid residues was 60,085. The N-terminal sequence and a partial internal amino acid sequence of purified H-450, which were determined chemically, were both found in the amino acid sequence of H-450 deduced from the nucleotide sequence. H-450 mRNA is expressed in liver, kidney, and brain. A homology search of amino acid sequences indicated that H-450 shows no homology with cytochrome P-450, but shows significant homology with bacterial O-acetylserine (thiol)-lyases. However, H-450 has no O-acetylserine (thiol)-lyase activity.

元の言語英語
ページ(範囲)899-902
ページ数4
ジャーナルJournal of biochemistry
108
発行部数6
DOI
出版物ステータス出版済み - 1 1 1990

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Cloning
Molecular Cloning
Liver
Sequence Analysis
Rats
Complementary DNA
Amino Acids
Cysteine Synthase
Amino Acid Sequence
Amino Acid Sequence Homology
Gene Library
Cytochrome P-450 Enzyme System
H 450
hemoprotein H-450
Brain
Nucleotides
Clone Cells
Molecular Weight
Molecular weight
Kidney

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

これを引用

Molecular cloning and sequence analysis of cDNA coding for rat liver hemoprotein H-450. / Ishihara, Satoru; Morohashi, Ken-Ichirou; Sadano, Hiroyuki; Kawabata, Shun-Ichiro; Gotoh, Osamu; Omura, Tsuneo.

:: Journal of biochemistry, 巻 108, 番号 6, 01.01.1990, p. 899-902.

研究成果: ジャーナルへの寄稿記事

Ishihara, Satoru ; Morohashi, Ken-Ichirou ; Sadano, Hiroyuki ; Kawabata, Shun-Ichiro ; Gotoh, Osamu ; Omura, Tsuneo. / Molecular cloning and sequence analysis of cDNA coding for rat liver hemoprotein H-450. :: Journal of biochemistry. 1990 ; 巻 108, 番号 6. pp. 899-902.
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