Molecular cloning and sequence analysis of cDNA coding for rat liver hemoprotein H-450

Satoru Ishihara, Ken ichirou Morohashi, Hiroyuki Sadano, Shun ichiro Kawabata, Osamu Gotoh, Tsuneo Omura

研究成果: Contribution to journalArticle査読

19 被引用数 (Scopus)

抄録

cDNA clones coding for hemoprotein H-450 were isolated from a rat liver cDNA library using anti-H-450 antibody. The molecular weight calculated from the deduced amino acid sequence comprising 547 amino acid residues was 60,085. The N-terminal sequence and a partial internal amino acid sequence of purified H-450, which were determined chemically, were both found in the amino acid sequence of H-450 deduced from the nucleotide sequence. H-450 mRNA is expressed in liver, kidney, and brain. A homology search of amino acid sequences indicated that H-450 shows no homology with cytochrome P-450, but shows significant homology with bacterial O-acetylserine (thiol)-lyases. However, H-450 has no O-acetylserine (thiol)-lyase activity.

本文言語英語
ページ(範囲)899-902
ページ数4
ジャーナルJournal of biochemistry
108
6
DOI
出版ステータス出版済み - 12 1990

All Science Journal Classification (ASJC) codes

  • 生化学
  • 分子生物学

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