TY - JOUR
T1 - Molecular cloning and tissue distribution of a receptor for pituitary adenylate cyclase-activating polypeptide
AU - Hashimoto, Hitoshi
AU - Ishihara, Takeshi
AU - Shigemoto, Ryuichi
AU - Mori, Kensaku
AU - Nagata, Shigekazu
N1 - Funding Information:
We are grateful to Drs. Y. Sugimoto, A. Ichikawa, J. Ogasawara, R. Fukunaga, H. Aino, and A. Baba for discussion and advice. We also thank Ms. K. Mimura for secretarial assistance. This workwas supported in part by the Ministry of Education, Science and Culture of Japan.
PY - 1993/8
Y1 - 1993/8
N2 - Pituitary adenylate cyclase-activating polypeptide (PACAP) is a polypeptide hormone related to vasoactive intestinal polypeptide (VIP). Rat PACAP receptor cDNA was isolated from a brain cDNA library by cross-hybridization with rat VIP receptor cDNA. The recombinant PACAP receptor expressed in COS cells bound PACAP with about 1000 times higher affinity than VIP, and PACAP stimulated adenylate cyclase through the cloned PACAP receptor. The rat PACAP receptor consists of 495 amino acids, contains seven transmembrane segments, and has a significant similarity with other Gs-coupled receptors, such as VIP, glucagon, and secretin receptors. PACAP receptor mRNA was abundantly expressed in the brain, but not in the peripheral tissues except for the adrenal gland. In situ hybridization revealed a high level of expression of PACAP receptor mRNA in the hippocampal dentate gyrus, olfactory bulb, and cerebellar cortex.
AB - Pituitary adenylate cyclase-activating polypeptide (PACAP) is a polypeptide hormone related to vasoactive intestinal polypeptide (VIP). Rat PACAP receptor cDNA was isolated from a brain cDNA library by cross-hybridization with rat VIP receptor cDNA. The recombinant PACAP receptor expressed in COS cells bound PACAP with about 1000 times higher affinity than VIP, and PACAP stimulated adenylate cyclase through the cloned PACAP receptor. The rat PACAP receptor consists of 495 amino acids, contains seven transmembrane segments, and has a significant similarity with other Gs-coupled receptors, such as VIP, glucagon, and secretin receptors. PACAP receptor mRNA was abundantly expressed in the brain, but not in the peripheral tissues except for the adrenal gland. In situ hybridization revealed a high level of expression of PACAP receptor mRNA in the hippocampal dentate gyrus, olfactory bulb, and cerebellar cortex.
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U2 - 10.1016/0896-6273(93)90188-W
DO - 10.1016/0896-6273(93)90188-W
M3 - Article
C2 - 8394723
AN - SCOPUS:0027317350
SN - 0896-6273
VL - 11
SP - 333
EP - 342
JO - Neuron
JF - Neuron
IS - 2
ER -