Molecular cloning, characterization and expression analysis of complement components in red sea bream (Pagrus major) after Edwardsiella tarda and red sea bream Iridovirus (RSIV) challenge

Mohammad Ali Noman Reza, Sipra Mohapatra, Sonoko Shimizu, Shin Ichi Kitamura, Shogo Harakawa, Hidemasa Kawakami, Kei Nakayama, Eitaro Sawayama, Takahiro Matsubara, Kohei Ohta, Tapas Chakraborty

研究成果: ジャーナルへの寄稿記事

抄録

The complement system plays an important role in immune regulation and acts as the first line of defense against any pathogenic attack. To comprehend the red sea bream (Pagrus major) immune response, three complement genes, namely, pmC1r, pmMASP and pmC3, belonging to the classical, lectin and alternative complement cascade, respectively, were identified and characterized. pmC1r, pmMASP, and pmC3 were comprised of 2535, 3352, and 5735 base mRNA which encodes 732, 1029 and 1677 aa putative proteins, respectively. Phylogenetically, all the three studied genes clustered with their corresponding homologous clade. Tissue distribution and cellular localization data demonstrated a very high prevalence of all the three genes in the liver. Both bacterial and viral infection resulted in significant transcriptional alterations in all three genes in the liver with respect to their vehicle control counterparts. Specifically, bacterial challenge affected the pmMASP and pmC3 expression, while the viral infection resulted in pmC1r and pmC3 mRNA activation. Altogether, our data demonstrate the ability of pmC1r, pmMASP and pmC3 in bringing about an immune response against any pathogenic encroachment, and thus activating, not only one, but all the three complement pathways, in red sea bream.

元の言語英語
ページ(範囲)286-295
ページ数10
ジャーナルFish and Shellfish Immunology
82
DOI
出版物ステータス出版済み - 11 1 2018

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Infectious spleen and kidney necrosis virus
Edwardsiella tarda
Pagrus major
Cloning
molecular cloning
complement
Genes
gene
immune response
Liver
genes
Messenger RNA
liver
tissue distribution
Lectins
infection
lectins
Chemical activation
Tissue
protein

All Science Journal Classification (ASJC) codes

  • Environmental Chemistry
  • Aquatic Science

これを引用

Molecular cloning, characterization and expression analysis of complement components in red sea bream (Pagrus major) after Edwardsiella tarda and red sea bream Iridovirus (RSIV) challenge. / Noman Reza, Mohammad Ali; Mohapatra, Sipra; Shimizu, Sonoko; Kitamura, Shin Ichi; Harakawa, Shogo; Kawakami, Hidemasa; Nakayama, Kei; Sawayama, Eitaro; Matsubara, Takahiro; Ohta, Kohei; Chakraborty, Tapas.

:: Fish and Shellfish Immunology, 巻 82, 01.11.2018, p. 286-295.

研究成果: ジャーナルへの寄稿記事

Noman Reza, Mohammad Ali ; Mohapatra, Sipra ; Shimizu, Sonoko ; Kitamura, Shin Ichi ; Harakawa, Shogo ; Kawakami, Hidemasa ; Nakayama, Kei ; Sawayama, Eitaro ; Matsubara, Takahiro ; Ohta, Kohei ; Chakraborty, Tapas. / Molecular cloning, characterization and expression analysis of complement components in red sea bream (Pagrus major) after Edwardsiella tarda and red sea bream Iridovirus (RSIV) challenge. :: Fish and Shellfish Immunology. 2018 ; 巻 82. pp. 286-295.
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abstract = "The complement system plays an important role in immune regulation and acts as the first line of defense against any pathogenic attack. To comprehend the red sea bream (Pagrus major) immune response, three complement genes, namely, pmC1r, pmMASP and pmC3, belonging to the classical, lectin and alternative complement cascade, respectively, were identified and characterized. pmC1r, pmMASP, and pmC3 were comprised of 2535, 3352, and 5735 base mRNA which encodes 732, 1029 and 1677 aa putative proteins, respectively. Phylogenetically, all the three studied genes clustered with their corresponding homologous clade. Tissue distribution and cellular localization data demonstrated a very high prevalence of all the three genes in the liver. Both bacterial and viral infection resulted in significant transcriptional alterations in all three genes in the liver with respect to their vehicle control counterparts. Specifically, bacterial challenge affected the pmMASP and pmC3 expression, while the viral infection resulted in pmC1r and pmC3 mRNA activation. Altogether, our data demonstrate the ability of pmC1r, pmMASP and pmC3 in bringing about an immune response against any pathogenic encroachment, and thus activating, not only one, but all the three complement pathways, in red sea bream.",
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AU - Mohapatra, Sipra

AU - Shimizu, Sonoko

AU - Kitamura, Shin Ichi

AU - Harakawa, Shogo

AU - Kawakami, Hidemasa

AU - Nakayama, Kei

AU - Sawayama, Eitaro

AU - Matsubara, Takahiro

AU - Ohta, Kohei

AU - Chakraborty, Tapas

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