Molecular cloning of a gene encoding matrix metalloproteinase-like protein from Gnathostoma spinigerum

P. Uparanukraw, N. Morakote, T. Harnnoi, A. Dantrakool

研究成果: ジャーナルへの寄稿学術誌査読

23 被引用数 (Scopus)

抄録

The advanced third-stage larvae (aL3) of Gnathostoma spinigerum contain a 24 kDa glycoprotein with diagnostic potential. Immunoscreening with the monoclonal antibody to the 24-kDa protein (mAb GN6/24) has identified a cDNA clone with an insert of 932 base pairs (bp). The insert contains a full-length gene of 732 bp encoding a protein that is 33-39% similar to matrix metalloproteinases (MMPs) of Caenorhabditis elegans and several lower and higher vertebrates. The MMP-like protein of G. spinigerum possesses the catalytic domain, but lacks the propeptide and hemopexin-like domains found in other MMPs. A signal peptide of 23 amino acids at its amino terminus indicates that it is a secretory protein, which is confirmed by Western blot analysis showing the presence of the 24 kDa protein in the excretory-secretory products of aL3.

本文言語英語
ページ(範囲)751-757
ページ数7
ジャーナルParasitology Research
87
9
DOI
出版ステータス出版済み - 8月 14 2001

!!!All Science Journal Classification (ASJC) codes

  • 寄生虫科
  • 獣医学(全般)
  • 昆虫科学
  • 感染症

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