Molecular cloning of CD4 from ginbuna crucian carp Carassius auratus langsdorfii

Seiko Nonaka, Tomonori Somamoto, Yoko Kato-Unoki, Mitsuru Ototake, Teruyuki Nakanishi, Miki Nakao

研究成果: ジャーナルへの寄稿記事

11 引用 (Scopus)

抄録

The clonal triploid ginbuna crucian carp Carassius auratus langsdorfii, a naturally occurring gynogenetic fish, is a useful model for studying T-cell-mediated immunity. CD4, a T-cell receptor (TCR) coreceptor, is a membrane-bound glycoprotein found on helper T-cells, and assists in the binding of major histocompatibility complexes. In the present study, full-length cDNAs encoding the CD4 molecule from the S3n strain of ginbuna crucian carp were cloned and characterized. 5′-rapid amplification of cDNA ends (RACE) and 3′-RACE yielded two distinct cDNA clones of CD4 homolog from the ginbuna, and these sequences share 95% identity at the amino acid level. These ginbuna CD4 molecules consisted of a signal peptide, immunoglobulin superfamily (IgSf) like domains, a transmembrane domain, and a cytoplasmic domain similar to other known CD4. A tyrosine protein kinase p56lck binding motif is conserved in the cytoplasmic tail of ginbuna CD4. Phylogenetic tree analysis indicated that ginbuna CD4 sequences are closely related to CD4L-1 from other fish species. Expression of ginbuna CD4 mRNA was detected in the gill, thymus, head kidney, trunk kidney and peripheral blood leukocytes, indicating that its expression pattern is similar to that of ginbuna TCRβ mRNA. The results suggest that ginbuna CD4 sequences are useful as molecular probes for helper T-cells.

元の言語英語
ページ(範囲)341-346
ページ数6
ジャーナルFisheries science
74
発行部数2
DOI
出版物ステータス出版済み - 4 1 2008

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Carassius
Carassius auratus
molecular cloning
amplification
T-lymphocytes
major histocompatibility complex
fish
immunity
peptide
rapid amplification of cDNA ends
clone
amino acid
blood
probe
membrane
phylogenetics
protein
kidneys
receptors
protein-tyrosine kinases

All Science Journal Classification (ASJC) codes

  • Aquatic Science

これを引用

Molecular cloning of CD4 from ginbuna crucian carp Carassius auratus langsdorfii. / Nonaka, Seiko; Somamoto, Tomonori; Kato-Unoki, Yoko; Ototake, Mitsuru; Nakanishi, Teruyuki; Nakao, Miki.

:: Fisheries science, 巻 74, 番号 2, 01.04.2008, p. 341-346.

研究成果: ジャーナルへの寄稿記事

Nonaka, Seiko ; Somamoto, Tomonori ; Kato-Unoki, Yoko ; Ototake, Mitsuru ; Nakanishi, Teruyuki ; Nakao, Miki. / Molecular cloning of CD4 from ginbuna crucian carp Carassius auratus langsdorfii. :: Fisheries science. 2008 ; 巻 74, 番号 2. pp. 341-346.
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