Molecular cloning of DAX1 and SHP cDNAs and their expression patterns in the Nile tilapia, Oreochromis niloticus

De Shou Wang, Tohru Kobayashi, Balasubramanian Senthilkumaran, Fumie Sakai, Cheni Chery Sudhakumari, Taiga Suzuki, Michiyasu Yoshikuni, Masaru Matsuda, Ken ichirou Morohashi, Yoshitaka Nagahama

研究成果: Contribution to journalArticle査読

51 被引用数 (Scopus)

抄録

Piscine DAX1 and SHP cDNAs with an open reading frame encoding 296 and 258 amino acid residues, respectively, as well as SHP partial gene fragment, were cloned from Nile tilapia. Phylogenetic analyses of DAX1s, SHPs, and homologous EST fragments indicate that DAX1 and SHP are conserved in gene structure and are present throughout vertebrates. A single band of ∼1.4 kb for DAX1 and of ∼1.2 kb for SHP was detected in the Northern blot analysis. Tissue distribution analysis by RT-PCR showed that fish DAX1 and SHP mRNAs are widely expressed in adult tissues, with the most abundant expression in gonads and liver, respectively. DAX1 and SHP were also detected in gonads of both sexes at 5-90 days after hatching (dah). However, the expression of DAX1 is weak at 5 and 10 dah and then significantly up-regulated between 10 and 15 dah, whereas the expression of SHP is moderate and consistent during the ontogeny.

本文言語英語
ページ(範囲)632-640
ページ数9
ジャーナルBiochemical and Biophysical Research Communications
297
3
DOI
出版ステータス出版済み - 2002
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

フィンガープリント

「Molecular cloning of DAX1 and SHP cDNAs and their expression patterns in the Nile tilapia, Oreochromis niloticus」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル