抄録
P450scc is the rate-limiting hormonally regulated enzyme that cleaves the cholesterol side chain. Translation of bovine adrenocortical mRNA and immunoprecipitation with rabbit anti-bovine P450scc indicates P450scc mRNA represents 1% of the total. DNA complementary to bovine adrenocortical mRNA was cloned in the PstI site of pBR322 by dC·dG tailing and high-efficiency transformation. A clone containing sequences complementary to P450scc mRNA was identified by hybrid-selected translation only when plasmid DNA was first purified by CsCl gradient centrifugation. As is often the case with hybrid-selected translation, the clone identified contains a small insert.
本文言語 | 英語 |
---|---|
ページ(範囲) | 264-270 |
ページ数 | 7 |
ジャーナル | Biochemical and Biophysical Research Communications |
巻 | 120 |
号 | 1 |
DOI | |
出版ステータス | 出版済み - 4月 16 1984 |
外部発表 | はい |
!!!All Science Journal Classification (ASJC) codes
- 生物理学
- 生化学
- 分子生物学
- 細胞生物学