Molecular cloning of DNA complementary to bovine adrenal P450scc mRNA

Karla J. Matteson; Bon chu Chung; Walter L. Miller

doi:10.1016/0006-291X(84)91443-8

Molecular cloning of DNA complementary to bovine adrenal P450_scc mRNA

Karla J. Matteson, Bon chu Chung, Walter L. Miller

研究成果: ジャーナルへの寄稿 › 学術誌 › 査読

11 被引用数 (Scopus)

抄録

P450_scc is the rate-limiting hormonally regulated enzyme that cleaves the cholesterol side chain. Translation of bovine adrenocortical mRNA and immunoprecipitation with rabbit anti-bovine P450_scc indicates P450_scc mRNA represents 1% of the total. DNA complementary to bovine adrenocortical mRNA was cloned in the PstI site of pBR322 by dC·dG tailing and high-efficiency transformation. A clone containing sequences complementary to P450_scc mRNA was identified by hybrid-selected translation only when plasmid DNA was first purified by CsCl gradient centrifugation. As is often the case with hybrid-selected translation, the clone identified contains a small insert.

本文言語	英語
ページ（範囲）	264-270
ページ数	7
ジャーナル	Biochemical and Biophysical Research Communications
巻	120
号	1
DOI	https://doi.org/10.1016/0006-291X(84)91443-8
出版ステータス	出版済み - 4月 16 1984
外部発表	はい

!!!All Science Journal Classification (ASJC) codes

生物理学
生化学
分子生物学
細胞生物学

文献へのアクセス

10.1016/0006-291X(84)91443-8

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引用スタイル

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title = "Molecular cloning of DNA complementary to bovine adrenal P450scc mRNA",

abstract = "P450scc is the rate-limiting hormonally regulated enzyme that cleaves the cholesterol side chain. Translation of bovine adrenocortical mRNA and immunoprecipitation with rabbit anti-bovine P450scc indicates P450scc mRNA represents 1% of the total. DNA complementary to bovine adrenocortical mRNA was cloned in the PstI site of pBR322 by dC·dG tailing and high-efficiency transformation. A clone containing sequences complementary to P450scc mRNA was identified by hybrid-selected translation only when plasmid DNA was first purified by CsCl gradient centrifugation. As is often the case with hybrid-selected translation, the clone identified contains a small insert.",

author = "Matteson, {Karla J.} and Chung, {Bon chu} and Miller, {Walter L.}",

note = "Funding Information: We thank Drs. Michael R. Waterman and Evan R. Siqson of the University of Texas Health Science Center in Dallas, who provided us with the rabbit anti-bovine P450 antibody without which we could not have done this work (supported by N#&ant AM 28350 to M.R.W.). We also thank John Fiddes for advice about the transformation procedure and Harald Biesmnann for his gift of --E. coli MM 294. Supported by NIH grant HD 16047 to W.L.M.",

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AU - Chung, Bon chu

AU - Miller, Walter L.

N1 - Funding Information: We thank Drs. Michael R. Waterman and Evan R. Siqson of the University of Texas Health Science Center in Dallas, who provided us with the rabbit anti-bovine P450 antibody without which we could not have done this work (supported by N#&ant AM 28350 to M.R.W.). We also thank John Fiddes for advice about the transformation procedure and Harald Biesmnann for his gift of --E. coli MM 294. Supported by NIH grant HD 16047 to W.L.M.

PY - 1984/4/16

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N2 - P450scc is the rate-limiting hormonally regulated enzyme that cleaves the cholesterol side chain. Translation of bovine adrenocortical mRNA and immunoprecipitation with rabbit anti-bovine P450scc indicates P450scc mRNA represents 1% of the total. DNA complementary to bovine adrenocortical mRNA was cloned in the PstI site of pBR322 by dC·dG tailing and high-efficiency transformation. A clone containing sequences complementary to P450scc mRNA was identified by hybrid-selected translation only when plasmid DNA was first purified by CsCl gradient centrifugation. As is often the case with hybrid-selected translation, the clone identified contains a small insert.

AB - P450scc is the rate-limiting hormonally regulated enzyme that cleaves the cholesterol side chain. Translation of bovine adrenocortical mRNA and immunoprecipitation with rabbit anti-bovine P450scc indicates P450scc mRNA represents 1% of the total. DNA complementary to bovine adrenocortical mRNA was cloned in the PstI site of pBR322 by dC·dG tailing and high-efficiency transformation. A clone containing sequences complementary to P450scc mRNA was identified by hybrid-selected translation only when plasmid DNA was first purified by CsCl gradient centrifugation. As is often the case with hybrid-selected translation, the clone identified contains a small insert.

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