Molecular cloning of DNA complementary to bovine adrenal P450scc mRNA

Karla J. Matteson, Bon chu Chung, Walter L. Miller

研究成果: Contribution to journalArticle査読

11 被引用数 (Scopus)

抄録

P450scc is the rate-limiting hormonally regulated enzyme that cleaves the cholesterol side chain. Translation of bovine adrenocortical mRNA and immunoprecipitation with rabbit anti-bovine P450scc indicates P450scc mRNA represents 1% of the total. DNA complementary to bovine adrenocortical mRNA was cloned in the PstI site of pBR322 by dC·dG tailing and high-efficiency transformation. A clone containing sequences complementary to P450scc mRNA was identified by hybrid-selected translation only when plasmid DNA was first purified by CsCl gradient centrifugation. As is often the case with hybrid-selected translation, the clone identified contains a small insert.

本文言語英語
ページ(範囲)264-270
ページ数7
ジャーナルBiochemical and Biophysical Research Communications
120
1
DOI
出版ステータス出版済み - 4 16 1984
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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