Molecular cloning of globotriaosylceramide/CD77 synthase, a glycosyltransferase that initiates the synthesis of globo series glycosphingolipids

Yoshinao Kojima, Satoshi Fukumoto, Keiko Furukawa, Tetsuya Okajima, Joelle Wiels, Keiko Yokoyama, Yasuo Suzuk, Takeshi Urano, Michio Ohta, Koichi Furukawa

研究成果: ジャーナルへの寄稿記事

114 引用 (Scopus)

抄録

The expression cloning of a cDNA for globotriaosylceramide (Gb3)/CD77 synthase (α1,4-galactosyltransferase) was achieved using an anti-Gb3 antibody and mouse L cells as a recipient cell line for the transfection. The isolated cDNA clone designated pVTR1 predicted a type II membrane protein with 19 amino acids of cytoplasmic domain, 26 amino acids of transmembrane region, and a catalytic domain with 308 amino acids. Introduction of the cDNA clone into L cells resulted in the neosynthesis of Gb3/CD77, and the extracts of the transfectant cells showed α1,4-galactosyltransferase activity only on lactosylceramide and galactosylceramide. In Northern blotting, a 2.3-kilobase mRNA was strongly expressed in heart, kidney, spleen, and placenta and weakly in colon, small intestine, and brain. Transfection of the cDNA into L cells resulted in the constitution of sensitivity to the apoptosis with Shiga-like toxins (verotoxins). Since Gb3/CD77 synthase initiates the synthesis of globo series glycolipids, the isolation of this cDNA will make possible further investigations into the function of its important series of glycolipids.

元の言語英語
ページ(範囲)15152-15156
ページ数5
ジャーナルJournal of Biological Chemistry
275
発行部数20
DOI
出版物ステータス出版済み - 5 19 2000
外部発表Yes

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Glycosphingolipids
Glycosyltransferases
Cloning
Molecular Cloning
Complementary DNA
Shiga Toxins
Galactosyltransferases
Glycolipids
Amino Acids
Transfection
Clone Cells
Galactosylceramides
Constitution and Bylaws
Cell Extracts
Northern Blotting
Placenta
Small Intestine
Organism Cloning
Anti-Idiotypic Antibodies
Brain

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

これを引用

Molecular cloning of globotriaosylceramide/CD77 synthase, a glycosyltransferase that initiates the synthesis of globo series glycosphingolipids. / Kojima, Yoshinao; Fukumoto, Satoshi; Furukawa, Keiko; Okajima, Tetsuya; Wiels, Joelle; Yokoyama, Keiko; Suzuk, Yasuo; Urano, Takeshi; Ohta, Michio; Furukawa, Koichi.

:: Journal of Biological Chemistry, 巻 275, 番号 20, 19.05.2000, p. 15152-15156.

研究成果: ジャーナルへの寄稿記事

Kojima, Y, Fukumoto, S, Furukawa, K, Okajima, T, Wiels, J, Yokoyama, K, Suzuk, Y, Urano, T, Ohta, M & Furukawa, K 2000, 'Molecular cloning of globotriaosylceramide/CD77 synthase, a glycosyltransferase that initiates the synthesis of globo series glycosphingolipids', Journal of Biological Chemistry, 巻. 275, 番号 20, pp. 15152-15156. https://doi.org/10.1074/jbc.M909620199
Kojima, Yoshinao ; Fukumoto, Satoshi ; Furukawa, Keiko ; Okajima, Tetsuya ; Wiels, Joelle ; Yokoyama, Keiko ; Suzuk, Yasuo ; Urano, Takeshi ; Ohta, Michio ; Furukawa, Koichi. / Molecular cloning of globotriaosylceramide/CD77 synthase, a glycosyltransferase that initiates the synthesis of globo series glycosphingolipids. :: Journal of Biological Chemistry. 2000 ; 巻 275, 番号 20. pp. 15152-15156.
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abstract = "The expression cloning of a cDNA for globotriaosylceramide (Gb3)/CD77 synthase (α1,4-galactosyltransferase) was achieved using an anti-Gb3 antibody and mouse L cells as a recipient cell line for the transfection. The isolated cDNA clone designated pVTR1 predicted a type II membrane protein with 19 amino acids of cytoplasmic domain, 26 amino acids of transmembrane region, and a catalytic domain with 308 amino acids. Introduction of the cDNA clone into L cells resulted in the neosynthesis of Gb3/CD77, and the extracts of the transfectant cells showed α1,4-galactosyltransferase activity only on lactosylceramide and galactosylceramide. In Northern blotting, a 2.3-kilobase mRNA was strongly expressed in heart, kidney, spleen, and placenta and weakly in colon, small intestine, and brain. Transfection of the cDNA into L cells resulted in the constitution of sensitivity to the apoptosis with Shiga-like toxins (verotoxins). Since Gb3/CD77 synthase initiates the synthesis of globo series glycolipids, the isolation of this cDNA will make possible further investigations into the function of its important series of glycolipids.",
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AU - Wiels, Joelle

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AU - Furukawa, Koichi

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