Molecular cloning of the genes involved in anthocyanin biosynthesis in Camellia japonica

Nobumine Tateishi, Yukio Ozaki, Hiroshi Okubo

研究成果: Contribution to journalArticle査読

抄録

Partial cDNA sequences of three anthocyanin biosynthetic genes (F3H, flavanone 3-hydroxylase; DFR, dihydroflavonol 4-reductase; ANS, anthocyanidin synthase) were isolated from the petals of Camellia japonica. Their deduced partial amino acid sequences shared high homologies with those of woody plant species (CjF3Ha, 98.0%, CjF3Hb, 91.2% and CjDFR, 99.0% with Camellia sinensis; CjANS, 90.3% with Rhododendron x pulchrum). Some important amino acid residues for enzymatic activities were also conserved in the isolated clones, suggesting that the genes we identified in this study were the homologues of C. japonica. Gene-specific primer pairs were designed based on each partial cDNA sequence. The application of these primer pairs to RT-PCR analyses was tested.

本文言語英語
ページ(範囲)21-28
ページ数8
ジャーナルJournal of the Faculty of Agriculture, Kyushu University
55
1
出版ステータス出版済み - 2 2010
外部発表はい

All Science Journal Classification (ASJC) codes

  • 農業および作物学
  • バイオテクノロジー

フィンガープリント

「Molecular cloning of the genes involved in anthocyanin biosynthesis in Camellia japonica」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル