Molecular crowding effect on enzymatic reaction in a fl-microchamber to mimic crowded intracellular environment

H. Murahara, N. Kaji, M. Tokeshi, Y. Baba

研究成果: 書籍/レポート タイプへの寄稿会議への寄与

2 被引用数 (Scopus)

抄録

In this work, we investigated how the size of reaction space would affect enzyme activities to understand intracellular enzyme kinetics. Two approaches were used to create pseudo-intracellular environments: a high concentration of polymers in bulk state and microfabricated chambers. Both approaches were carefully compared and we found that an enzyme activity was inhibited in 7.2 fL chamber by molecular crowding effect as in the case of highly concentrated polymer solution.

本文言語英語
ホスト出版物のタイトルProceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007
編集者Jean-Louis Viovy, Patrick Tabeling, Stephanie Descroix, Laurent Malaquin
出版社Chemical and Biological Microsystems Society
ページ1003-1005
ページ数3
ISBN(電子版)9780979806407
出版ステータス出版済み - 2007
外部発表はい
イベント11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007 - Paris, フランス
継続期間: 10月 7 200710月 11 2007

出版物シリーズ

名前Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007

会議

会議11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007
国/地域フランス
CityParis
Period10/7/0710/11/07

!!!All Science Journal Classification (ASJC) codes

  • 化学工学(その他)
  • バイオエンジニアリング

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