Molecular evolution of prepro-thyrotropin-releasing hormone in the chicken (Gallus gallus) and its expression in the brain

Yasuhiro Aoki, Hiroko Ono, Shinobu Yasuo, Tomohiro Masuda, Takashi Yoshimura, Shizufumi Ebihara, Masayuki Iigo, Tadashi Yanagisawa

研究成果: Contribution to journalArticle査読

8 被引用数 (Scopus)

抄録

A cDNA encoding prepro-thyrotropin-relaesing hormone (ppTRH) in chicken (Callus gallus) was isolated and the sites of expression in the brain were determined. The chicken ppTRH cDNA encodes 260 amino acids, including four TRH progenitor sequences (-Lys/Arg-Arg-Gln-His-Pro-Gly-Lys/Arg-Arg-). It is interesting to note that chicken ppTRH harbors four TRH progenitor-like sequences. According to the hydropathy profile of chicken ppTRH, not only the TRH progenitor sequences but also the TRH progenitor-like sequences are localized in hydrophilic regions. The TRH progenitor-like sequences might be related to structural conservation in the evolution of ppTRH, although they cannot be processed into TRH due to the mutation of several amino acids. According to the alignment of the deduced amino-acid sequences of known vertebrate ppTRHs and the molecular phylogenetic tree we constructed, we speculate on the molecular evolution of ppTRH in vertebrates. In situ hybridization demonstrated experession of the ppTRH gene in the nucleus preopticus periventricularis, nucleus preopticus medialis, regio lateralis hypothalami, paraventricular nucleus, nucleus periventricularis hypothalami, and nucleus ventromedialis hypothalami in the chicken brain.

本文言語英語
ページ(範囲)686-692
ページ数7
ジャーナルZoological science
24
7
DOI
出版ステータス出版済み - 7 1 2007
外部発表はい

All Science Journal Classification (ASJC) codes

  • Animal Science and Zoology

フィンガープリント 「Molecular evolution of prepro-thyrotropin-releasing hormone in the chicken (Gallus gallus) and its expression in the brain」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

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