Molecular imaging reveals elevated VEGFR-2 expression in retinal capillaries in diabetes: A novel biomarker for early diagnosis

Dawei Sun, Shintaro Nakao, Fang Xie, Souska Zandi, Abouzar Bagheri, Mozhgan Rezaei Kanavi, Shahram Samiei, Zahra Soheila Soheili, Sonja Frimmel, Zhongyu Zhang, Zsolt Ablonczy, Hamid Ahmadieh, Ali Hafezi-Moghadam

研究成果: ジャーナルへの寄稿学術誌査読

17 被引用数 (Scopus)

抄録

Diabetic retinopathy (DR) is a microvascular complication of diabetes and a leading cause of vision loss. Biomarkers and methods for early diagnosis of DR are urgently needed. Using a new molecular imaging approach, we show up to 94% higher accumulation of custom designed imaging probes against vascular endothelial growth factor receptor 2 (VEGFR-2) in retinal and choroidal vessels of diabetic animals (P<0.01), compared to normal controls. More than 80% of the VEGFR-2 in the diabetic retina was in the capillaries, compared to 47% in normal controls (P<0.01). Angiography in rabbit retinas revealed microvascular capillaries to be the location for VEGF-A-induced leakage, as expressed by significantly higher rate of fluorophore spreading with VEGF-A injection when compared to vehicle control (26±2 vs. 3±1 μm/s, P<0.05). Immunohistochemistry showed VEGFR-2 expression in capillaries of diabetic animals but not in normal controls. Macular vessels from diabetic patients (n=7) showed significantly more VEGFR-2 compared to nondiabetic controls (n=5) or peripheral retinal regions of the same retinas (P<0.01 in both cases). Here we introduce a new approach for early diagnosis of DR and VEGFR-2 as a molecular marker. VEGFR-2 could become a key diagnostic target, one that might help to prevent retinal vascular leakage and proliferation in diabetic patients.

本文言語英語
ページ(範囲)3942-3951
ページ数10
ジャーナルFASEB Journal
28
9
DOI
出版ステータス出版済み - 9月 1 2014

!!!All Science Journal Classification (ASJC) codes

  • バイオテクノロジー
  • 生化学
  • 分子生物学
  • 遺伝学

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