Molecular screening of both the promoter and the protein coding regions in the human ob gene in Japanese obese subjects with non-insulin-dependent diabetes mellitus

M. Shigemoto, S. Nishi, Y. Ogawa, N. Isse, N. Matsuoka, T. Tanaka, N. Azuma, H. Masuzaki, H. Nishimura, Y. Yoshimasa, K. Hosoda, K. Nakao

研究成果: Contribution to journalArticle査読

17 被引用数 (Scopus)

抄録

Objective: Although the molecular mechanism of obesity has been poorly understood, recent studies indicate that leptin plays a critical role in regulating both food intake and body weight. Because obesity decreases the sensitivity to insulin, the human ob gene is presumed to be one of the candidate genes for non-insulin-dependent diabetes mellitus (NIDDM) associated with obesity. Although the protein coding region in the ob gene has been screened for mutations, the promoter region and the noncoding first exon have not yet been studied. We investigated the involvement of the human ob gene, especially mutations at the promoter region and the non-coding first exon, in the development of NIDDM associated with obesity. Subjects: The study group comprised 60 Japanese obese subjects with NIDDM (body mass index (BMI) 43.6≤BMI≤26.4, 29.0±0.41 (mean±S.E.M.)) and 24 obese individuals with impaired glucose tolerance (IGT) (30≤BMI≤26.4, 27.1±0.22). Methods: Mutations at both the promoter region and all three exons in the human ob gene were screened by the single-stranded conformational polymorphism analysis. When aberrantly migrated bands were recognized, the PCR-amplified DNA fragment was directly sequenced. Results: In the protein coding region a silent mutation in the second exon was detected. The noncoding first exon and the about 100 bp 5'-flanking region of the gene which contains a proximal CCAAT/enhancer-binding protein site were screened, but no mutations were found. Conclusion: These results suggest that no mutations in either the promoter region at the about 100 bp 5'-flanking region of the gene, or in any of the three exons, are involved in the development of NIDDM or IGT associated with obesity.

本文言語英語
ページ(範囲)511-513
ページ数3
ジャーナルEuropean journal of endocrinology
137
5
DOI
出版ステータス出版済み - 11 1997
外部発表はい

All Science Journal Classification (ASJC) codes

  • 内分泌学、糖尿病および代謝内科学
  • 内分泌学

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