Molecular structure of a 5,10-methylenetetrahydrofolate dehydrogenase from the silkworm Bombyx mori

Mohammad R. Haque, Akifumi Higashiura, Atsushi Nakagawa, Aiko Hirowatari, Shigeki Furuya, Kohji Yamamoto

研究成果: ジャーナルへの寄稿記事

抄録

The enzyme 5,10-methylenetetrahydrofolate dehydrogenase (MTHFD) is essential for the production of certain amino acids (glycine, serine, and methionine) and nucleic acids (thymidylate and purine). Here, we identified a cDNA encoding this enzyme from the silkworm Bombyx mori. The recombinant B. mori MTHFD (bmMTHFD) expressed in Escherichia coli recognized 5,10-methylenetetrahydrofolate and 5,10-methenyltetrahydrofolate as substrate in the presence of NADP + as well as NAD + . The bmMTHFD structure was determined at a resolution of 1.75 Å by X-ray crystallography. Site-directed mutagenesis indicated that the amino acid residue Tyr49 contributed to its catalytic activity. Our findings provide insight into the mechanism underlying the activity of MTHFD from B. mori and potentially other insects and may therefore facilitate the development of inhibitors specific to MTHFD as insecticides.

元の言語英語
ページ(範囲)618-628
ページ数11
ジャーナルFEBS Open Bio
9
発行部数4
DOI
出版物ステータス出版済み - 4 1 2019

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Methylenetetrahydrofolate Dehydrogenase (NADP)
Bombyx
Molecular Structure
Morus
Molecular structure
Amino Acids
Mutagenesis
X ray crystallography
X Ray Crystallography
Enzymes
Insecticides
Site-Directed Mutagenesis
NADP
Methionine
NAD
Glycine
Serine
Nucleic Acids
Insects
Catalyst activity

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

これを引用

Molecular structure of a 5,10-methylenetetrahydrofolate dehydrogenase from the silkworm Bombyx mori. / Haque, Mohammad R.; Higashiura, Akifumi; Nakagawa, Atsushi; Hirowatari, Aiko; Furuya, Shigeki; Yamamoto, Kohji.

:: FEBS Open Bio, 巻 9, 番号 4, 01.04.2019, p. 618-628.

研究成果: ジャーナルへの寄稿記事

Haque, Mohammad R. ; Higashiura, Akifumi ; Nakagawa, Atsushi ; Hirowatari, Aiko ; Furuya, Shigeki ; Yamamoto, Kohji. / Molecular structure of a 5,10-methylenetetrahydrofolate dehydrogenase from the silkworm Bombyx mori. :: FEBS Open Bio. 2019 ; 巻 9, 番号 4. pp. 618-628.
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