Monitor of the myostatin autocrine action during differentiation of embryonic chicken myoblasts into myotubes: Effect of IGF-I

Masatoshi Kurokawa, Fuminori Sato, Shinya Aramaki, Tomoki Soh, Nobuhiko Yamauchi, Masa Aki Hattori

研究成果: Contribution to journalArticle査読

7 被引用数 (Scopus)

抄録

Myogenesis is regulated through the proliferation and differentiation of myoblasts expressing myostatin which functions as a negative regulator by generating Smad signals. Here, we monitored the autocrine action of myostatin in quiescent chicken myoblasts transfected with the Smad-mediated promoter reporter vector to evaluate the modulation of several growth factors. During differentiation of myoblasts into myotubes, stretched and spherical types of myoblasts were observed at 12 h after induction, at which the promoter activity began to increase. Maximal promoter activity was observed at approximately 30 h. Multinucleated myotubes were markedly formed at 72 h, but the activity was very low. IGF-I, known as a positive regulator of myogenesis, increased the promoter activity, but the increase was rather small at its high concentration (100 ng/ml). IGF-I significantly increased the level of myostatin transcript in myoblasts and newly formed myotubes at 24 h, but not at 36 h. However, the cell fusion of myoblasts was not accelerated in the presence of IGF-I. Consequently, this study indicates that the autocrine action of myostatin is partially enhanced by IGF-I through increasing its expression.

本文言語英語
ページ(範囲)193-199
ページ数7
ジャーナルMolecular and cellular biochemistry
331
1-2
DOI
出版ステータス出版済み - 2009

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology

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