Monitoring of the multiple bacteriocin production by Enterococcus faecium NKR-5-3 through a developed liquid chromatography and mass spectrometry-based quantification system

Rodney H. Perez, Kohei Himeno, Naoki Ishibashi, Yoshimitsu Masuda, Takeshi Zendo, Koji Fujita, Pongtep Wilaipun, Vichien Leelawatcharamas, Jiro Nakayama, Kenji Sonomoto

研究成果: ジャーナルへの寄稿記事

17 引用 (Scopus)

抄録

Enterococcus faecium NKR-5-3 produces four antimicrobial peptides referred here as enterocins NKR-5-3A, B, C and D. A two-step electrospray ionization-liquid chromatography and mass spectrometry (ESI-LC/MS)-based quantification system was developed to monitor its multiple bacteriocin production profiles, which is essential in understanding the complex production regulation mechanism of strain NKR-5-3. The developed ESI-LC/MS-based quantification system can easily monitor the multiple bacteriocin production of this strain. Using the developed system, the production of enterocin NKR-5-3B was found to be not as variable as those of the other enterocins in different cultivation media. Production of enterocin NKR-5-3B was also found to have a wider optimum incubation temperature (20-30°C) than enterocins NKR-5-3A, C and D (25°C). Furthermore, at least 2 nM of the bacteriocin-like inducing peptide, enterocin NKR-5-3D, regulated the production of NKR-5-3 enterocins except enterocin NKR-5-3B. These findings taken together suggest that enterocin NKR-5-3B has an independent production regulation mechanism from the other NKR-5-3 enterocins. The developed system could effectively pin-point the production profiles of the multiple bacteriocins of E. faecium NKR-5-3 under different fermentation conditions.

元の言語英語
ページ(範囲)490-496
ページ数7
ジャーナルJournal of Bioscience and Bioengineering
114
発行部数5
DOI
出版物ステータス出版済み - 11 1 2012

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Enterococcus faecium
Bacteriocins
Liquid chromatography
Liquid Chromatography
Mass spectrometry
Mass Spectrometry
Monitoring
Electrospray ionization
Peptides
enterocin
Fermentation

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

これを引用

Monitoring of the multiple bacteriocin production by Enterococcus faecium NKR-5-3 through a developed liquid chromatography and mass spectrometry-based quantification system. / Perez, Rodney H.; Himeno, Kohei; Ishibashi, Naoki; Masuda, Yoshimitsu; Zendo, Takeshi; Fujita, Koji; Wilaipun, Pongtep; Leelawatcharamas, Vichien; Nakayama, Jiro; Sonomoto, Kenji.

:: Journal of Bioscience and Bioengineering, 巻 114, 番号 5, 01.11.2012, p. 490-496.

研究成果: ジャーナルへの寄稿記事

Perez, Rodney H. ; Himeno, Kohei ; Ishibashi, Naoki ; Masuda, Yoshimitsu ; Zendo, Takeshi ; Fujita, Koji ; Wilaipun, Pongtep ; Leelawatcharamas, Vichien ; Nakayama, Jiro ; Sonomoto, Kenji. / Monitoring of the multiple bacteriocin production by Enterococcus faecium NKR-5-3 through a developed liquid chromatography and mass spectrometry-based quantification system. :: Journal of Bioscience and Bioengineering. 2012 ; 巻 114, 番号 5. pp. 490-496.
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