Monitoring RhoGDI extraction of lipid-modified Rho GTPases from membranes using click chemistry

Akiyuki Nishimura, Maurine E. Linder

研究成果: Chapter in Book/Report/Conference proceedingChapter

1 被引用数 (Scopus)

抄録

The posttranslational lipid modification of Rho GTPases is important for their proper subcellular localization and signal transduction. Rho GTPases terminate in a CaaX motif, in which the cysteine residue is modified with either a farnesyl or geranylgeranyl isoprenoid. RhoGDI renders Rho GTPases soluble by masking their lipid moieties. We recently identified that the brain-specific splice variant of Cdc42 (bCdc42) containing a noncanonical CCaX motif harbors a dual prenyl–palmitoyl modification that prevents its binding to RhoGDI. This chapter describes a method to analyze RhoGDI extraction of Rho GTPases containing different lipid modifications from membranes using a liposome reconstitution assay and click chemistry.

本文言語英語
ホスト出版物のタイトルMethods in Molecular Biology
出版社Humana Press Inc.
ページ297-306
ページ数10
DOI
出版ステータス出版済み - 2019
外部発表はい

出版物シリーズ

名前Methods in Molecular Biology
2009
ISSN(印刷版)1064-3745
ISSN(電子版)1940-6029

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

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