Monocyte chemoattractant protein 1 mediates retinal detachment-induced photoreceptor apoptosis

Toru Nakazawa, Toshio Hisatomi, Chifuyu Nakazawa, Kosuke Noda, Kazuichi Maruyama, Haicheng She, Akihisa Matsubara, Shinsuke Miyahara, Shintaro Nakao, Yuqin Yin, Larry Benowitz, Ali Hafezi-Moghadam, Joan W. Miller

研究成果: ジャーナルへの寄稿記事

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Photoreceptor apoptosis is a major cause of visual loss in retinal detachment (RD) and several other visual disorders, but the underlying mechanisms remain elusive. Recently, increased expression of monocyte chemoattractant protein 1 (MCP-1) was reported in vitreous humor samples of patients with RD and diabetic retinopathy as well as in the brain tissues of patients with neurodegenerative diseases, including Alzheimer's disease and multiple sclerosis. Here we report that MCP-1 plays a critical role in mediating photoreceptor apoptosis in an experimental model of RD. RD led to increased MCP-1 expression in the Müller glia and increased CD11b+ macrophage/ microglia in the detached retina. An MCP-1 blocking antibody greatly reduced macrophage/microglia infiltration and RD-induced photoreceptor apoptosis. Confirming these results, MCP-1 gene-deficient mice showed significantly reduced macrophage/microglia infiltration after RD and very little photoreceptor apoptosis. In primary retinal mixed cultures, MCP-1 was cytotoxic for recoverin+ photoreceptors, and this toxicity was eliminated through immunodepleting macrophage/microglia from the culture. In vivo, deletion of the gene encoding CD11b/CD18 nearly eliminated macrophage/microglia infiltration to the retina after RD and the loss of photoreceptors. Thus, MCP-1 expression and subsequent macrophage/microglia infiltration and activation are critical for RD-induced photoreceptor apoptosis. This pathway may be an important therapeutic target for preventing photoreceptor apoptosis in RD and other CNS diseases that share a common etiology.

元の言語英語
ページ(範囲)2425-2430
ページ数6
ジャーナルProceedings of the National Academy of Sciences of the United States of America
104
発行部数7
DOI
出版物ステータス出版済み - 2 13 2007

All Science Journal Classification (ASJC) codes

  • General

フィンガープリント Monocyte chemoattractant protein 1 mediates retinal detachment-induced photoreceptor apoptosis' の研究トピックを掘り下げます。これらはともに一意のフィンガープリントを構成します。

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    Nakazawa, T., Hisatomi, T., Nakazawa, C., Noda, K., Maruyama, K., She, H., Matsubara, A., Miyahara, S., Nakao, S., Yin, Y., Benowitz, L., Hafezi-Moghadam, A., & Miller, J. W. (2007). Monocyte chemoattractant protein 1 mediates retinal detachment-induced photoreceptor apoptosis. Proceedings of the National Academy of Sciences of the United States of America, 104(7), 2425-2430. https://doi.org/10.1073/pnas.0608167104