Monocyte-derived dendritic cells that capture dead tumor cells secrete IL-12 and TNF-α through IL-12/TNF-α/NF-κB autocrine loop

Hideya Ohnishi, Hideo Kuroki, Kotaro Matsumoto, Eishi Baba, Nobuhiko Sasaki, Hirotaka Kuga, Masao Tanaka, Mitsuo Katano, Takashi Morisaki

研究成果: ジャーナルへの寄稿記事

18 引用 (Scopus)

抄録

This study focused on the question of how monocyte-derived dendritic cells (Mo-DCs) that capture dead tumor cells (Mo-DCs-Tum) secrete interleukin 12 (IL-12) and tumor necrosis factor α (TNF-α). Mo-DCs-Tum showed higher secretions of IL-12 and TNF-α than were shown by Mo-DCs. Enhanced nuclear factor-kappa B (NF-κB) activation was also induced in MoDCs-Tum within 6 h. The NF-κB inhibitor, pyrrolidine dithiocarbamate (PDTC), suppressed both IL-12 and TNF-α secretions from Mo-DCs-Tum. Administration of recombinant TNF-α or IL-12 enhanced IL-12 or TNF-α secretion respectively in Mo-DCs-Tum. Addition of anti-TNF-α or anti-IL-12 neutralizing antibody decreased NF-κB activation and IL-12 or TNF-α secretion in Mo-DCs-Tum. These results suggest that TNF-α or IL-12 secretion induces NF-κB activation, and it stimulates further TNF-α and IL-12 secretions, i.e., an IL-12/TNF-α/NF-κB autocrine loop, in Mo-DCs-Tum. Thus, Mo-DCs-Tum secrete a large amount of IL-12 and TNF-α through accelerated NF-κB activation induced by the IL-12/TNF-α/NF- κB autocrine loop.

元の言語英語
ページ(範囲)1093-1100
ページ数8
ジャーナルCancer Immunology, Immunotherapy
53
発行部数12
DOI
出版物ステータス出版済み - 12 1 2004

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NF-kappa B
Interleukin-12
Dendritic Cells
Monocytes
Neoplasms
Neutralizing Antibodies
Tumor Necrosis Factor-alpha

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Oncology
  • Cancer Research

これを引用

Monocyte-derived dendritic cells that capture dead tumor cells secrete IL-12 and TNF-α through IL-12/TNF-α/NF-κB autocrine loop. / Ohnishi, Hideya; Kuroki, Hideo; Matsumoto, Kotaro; Baba, Eishi; Sasaki, Nobuhiko; Kuga, Hirotaka; Tanaka, Masao; Katano, Mitsuo; Morisaki, Takashi.

:: Cancer Immunology, Immunotherapy, 巻 53, 番号 12, 01.12.2004, p. 1093-1100.

研究成果: ジャーナルへの寄稿記事

Ohnishi, Hideya ; Kuroki, Hideo ; Matsumoto, Kotaro ; Baba, Eishi ; Sasaki, Nobuhiko ; Kuga, Hirotaka ; Tanaka, Masao ; Katano, Mitsuo ; Morisaki, Takashi. / Monocyte-derived dendritic cells that capture dead tumor cells secrete IL-12 and TNF-α through IL-12/TNF-α/NF-κB autocrine loop. :: Cancer Immunology, Immunotherapy. 2004 ; 巻 53, 番号 12. pp. 1093-1100.
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abstract = "This study focused on the question of how monocyte-derived dendritic cells (Mo-DCs) that capture dead tumor cells (Mo-DCs-Tum) secrete interleukin 12 (IL-12) and tumor necrosis factor α (TNF-α). Mo-DCs-Tum showed higher secretions of IL-12 and TNF-α than were shown by Mo-DCs. Enhanced nuclear factor-kappa B (NF-κB) activation was also induced in MoDCs-Tum within 6 h. The NF-κB inhibitor, pyrrolidine dithiocarbamate (PDTC), suppressed both IL-12 and TNF-α secretions from Mo-DCs-Tum. Administration of recombinant TNF-α or IL-12 enhanced IL-12 or TNF-α secretion respectively in Mo-DCs-Tum. Addition of anti-TNF-α or anti-IL-12 neutralizing antibody decreased NF-κB activation and IL-12 or TNF-α secretion in Mo-DCs-Tum. These results suggest that TNF-α or IL-12 secretion induces NF-κB activation, and it stimulates further TNF-α and IL-12 secretions, i.e., an IL-12/TNF-α/NF-κB autocrine loop, in Mo-DCs-Tum. Thus, Mo-DCs-Tum secrete a large amount of IL-12 and TNF-α through accelerated NF-κB activation induced by the IL-12/TNF-α/NF- κB autocrine loop.",
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AU - Matsumoto, Kotaro

AU - Baba, Eishi

AU - Sasaki, Nobuhiko

AU - Kuga, Hirotaka

AU - Tanaka, Masao

AU - Katano, Mitsuo

AU - Morisaki, Takashi

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