TY - JOUR
T1 - Morphological changes in rabbit corneal endothelium after surgical injuries
AU - Miyazaki, Masanori
AU - Tanaka, Toshiro
AU - Nishida, Teruo
PY - 1999/5/1
Y1 - 1999/5/1
N2 - Purpose: To understand the responses of the corneal endothelium to different types of surgery, we chronologically investigated the morphologic changes in the endothelial cells. Methods: We did a mechanical incision, epithelial ablation, and excimer laser radiation on rabbit corneas and observed the morphologic changes in the endothelial cells for up to 2 weeks after surgery under a light microscope and an electron microscope. Results: Although we observed enlarged intercellular spaces between neighboring endothelial cells, intercellular adhesion kept the cells tightly joined near their apexes immediately after each procedure. No signs of endothelial cell degeneration were observed after the procedures, but we did observe many Golgi apparati, rough-surfaced endoplasmic reticula, and secreted granules, indicating that the cells had been activated. After each procedure, the intercellular junctions and spaces required different amounts of time to return to normal. Conclusions: These results suggest that the different kinds of surgical injuries affected the corneal endothelium but that the changes were reversible.
AB - Purpose: To understand the responses of the corneal endothelium to different types of surgery, we chronologically investigated the morphologic changes in the endothelial cells. Methods: We did a mechanical incision, epithelial ablation, and excimer laser radiation on rabbit corneas and observed the morphologic changes in the endothelial cells for up to 2 weeks after surgery under a light microscope and an electron microscope. Results: Although we observed enlarged intercellular spaces between neighboring endothelial cells, intercellular adhesion kept the cells tightly joined near their apexes immediately after each procedure. No signs of endothelial cell degeneration were observed after the procedures, but we did observe many Golgi apparati, rough-surfaced endoplasmic reticula, and secreted granules, indicating that the cells had been activated. After each procedure, the intercellular junctions and spaces required different amounts of time to return to normal. Conclusions: These results suggest that the different kinds of surgical injuries affected the corneal endothelium but that the changes were reversible.
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M3 - Article
C2 - 10363508
AN - SCOPUS:0033025426
VL - 103
SP - 350
EP - 355
JO - Journal of Japanese Ophthalmological Society
JF - Journal of Japanese Ophthalmological Society
SN - 0029-0203
IS - 5
ER -