抄録
Recently, micro-nano fabrication has become an important way to modify material's surfaces for reguration of adhesion and growth of cells in tissue engineering. In neuroscience, micro-patterns on culture substrates are critical for neurological implants and are also significant to investigate neuron-patterned surface interactions. In previous studies, the patterns have been fabricated by lithography and microcontact printing techniques. However, these techniques need high energy and many processes and restricted can only treat a list of materials for substrates. In this study, we prepared self-organized honeycomb-patterned films by a casting technique. We cultured neurons on the patterned films for neural tissue engineering and investigated the morphology of neural cell bodies and neural network by a confocal scanning microscope and a scanning electron microscope. We found that neurons formed neural network along the honeycomb patterns and that the morphologies of attached cells depended on pore size of the patterned films. These results suggest that honeycomb-patterned films provide guidance of neurite extension and influence the morphologies of neurons.
| 元の言語 | 英語 |
|---|---|
| ページ（範囲） | 628-633 |
| ページ数 | 6 |
| ジャーナル | KOBUNSHI RONBUNSHU |
| 巻 | 61 |
| 発行部数 | 12 |
| DOI | |
| 出版物ステータス | 出版済み - 1 1 2004 |
| 外部発表 | Yes |
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All Science Journal Classification (ASJC) codes
- Chemical Engineering (miscellaneous)
- Materials Science (miscellaneous)
- Environmental Science(all)
- Polymers and Plastics
これを引用
Morphological changes of neurons on self-organized honeycomb patterned films. / Tsuruma, Akinori; Tanaka, Masaru; Fukushima, Nobuyuki; Shimomura, Masatsugu.
：: KOBUNSHI RONBUNSHU, 巻 61, 番号 12, 01.01.2004, p. 628-633.研究成果: ジャーナルへの寄稿 › 記事
}
TY - JOUR
T1 - Morphological changes of neurons on self-organized honeycomb patterned films
AU - Tsuruma, Akinori
AU - Tanaka, Masaru
AU - Fukushima, Nobuyuki
AU - Shimomura, Masatsugu
PY - 2004/1/1
Y1 - 2004/1/1
N2 - Recently, micro-nano fabrication has become an important way to modify material's surfaces for reguration of adhesion and growth of cells in tissue engineering. In neuroscience, micro-patterns on culture substrates are critical for neurological implants and are also significant to investigate neuron-patterned surface interactions. In previous studies, the patterns have been fabricated by lithography and microcontact printing techniques. However, these techniques need high energy and many processes and restricted can only treat a list of materials for substrates. In this study, we prepared self-organized honeycomb-patterned films by a casting technique. We cultured neurons on the patterned films for neural tissue engineering and investigated the morphology of neural cell bodies and neural network by a confocal scanning microscope and a scanning electron microscope. We found that neurons formed neural network along the honeycomb patterns and that the morphologies of attached cells depended on pore size of the patterned films. These results suggest that honeycomb-patterned films provide guidance of neurite extension and influence the morphologies of neurons.
AB - Recently, micro-nano fabrication has become an important way to modify material's surfaces for reguration of adhesion and growth of cells in tissue engineering. In neuroscience, micro-patterns on culture substrates are critical for neurological implants and are also significant to investigate neuron-patterned surface interactions. In previous studies, the patterns have been fabricated by lithography and microcontact printing techniques. However, these techniques need high energy and many processes and restricted can only treat a list of materials for substrates. In this study, we prepared self-organized honeycomb-patterned films by a casting technique. We cultured neurons on the patterned films for neural tissue engineering and investigated the morphology of neural cell bodies and neural network by a confocal scanning microscope and a scanning electron microscope. We found that neurons formed neural network along the honeycomb patterns and that the morphologies of attached cells depended on pore size of the patterned films. These results suggest that honeycomb-patterned films provide guidance of neurite extension and influence the morphologies of neurons.
UR - http://www.scopus.com/inward/record.url?scp=14044252061&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=14044252061&partnerID=8YFLogxK
U2 - 10.1295/koron.61.628
DO - 10.1295/koron.61.628
M3 - Article
AN - SCOPUS:14044252061
VL - 61
SP - 628
EP - 633
JO - Kobunshi Ronbunshu
JF - Kobunshi Ronbunshu
SN - 0386-2186
IS - 12
ER -