Morphological studies of the goldfish hindgut mucosa in organ culture

Hiroshi Iida, Torao Yamamoto

研究成果: ジャーナルへの寄稿記事

10 引用 (Scopus)

抄録

The morphology of the absorptive cells of the goldfish hindgut mucosa, and their capability for horseradish peroxidase (HRP) uptake, were investigated by electron microscopy after a 24-h organ culture. The columnar appearance and the fine structure of the absorptive cells were well preserved for 24 h at room temperature and 37° C with 5% CO2 in air, in all the media used in this study. Mitoses were frequently observed in the epithelium at the bottom of cultured mucosal folds, and re-epithelization was also observed in many explants. Some structural changes were, however, noted in the cultured absorptive cells, as compared with the non-cultured absorptive cells; the deep invaginations of the surface membrane between the microvilli decreased in number; supranuclear giant vacuoles were reduced in size or almost disappeared; the distributional pattern of mitochondria in the absorptive cells was altered. The HRP uptake experiments showed that the absorptive cells cultured for 24 h could still take up HRP by endocytosis and transport it, indicating that the absorptive cells maintained their capability of macromolecule uptake and transport after 24 h of culture. In addition, HRP experiments, in which reaction product was detected within numerous cytoplasmic tubules (CT), various vacuoles and CT-vacuole complexes, suggested a close relationship between CT and vacuolar system in the apical cytoplasm during endocytotic events in the absorptive cells.

元の言語英語
ページ(範囲)523-528
ページ数6
ジャーナルCell and Tissue Research
238
発行部数3
DOI
出版物ステータス出版済み - 11 1 1984

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Goldfish
Organ Culture Techniques
Mucous Membrane
Horseradish Peroxidase
Vacuoles
Cultured Cells
Microvilli
Endocytosis
Mitosis
Electron Microscopy
Mitochondria
Cytoplasm
Epithelium
Air
Temperature
Membranes

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

これを引用

Morphological studies of the goldfish hindgut mucosa in organ culture. / Iida, Hiroshi; Yamamoto, Torao.

:: Cell and Tissue Research, 巻 238, 番号 3, 01.11.1984, p. 523-528.

研究成果: ジャーナルへの寄稿記事

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