Mouse soleus (slow) muscle shows greater intramyocellular lipid droplet accumulation than EDL (fast) muscle

fiber type-specific analysis

Yusuke Komiya, Shoko Sawano, Daisuke Mashima, Riho Ichitsubo, Mako Nakamura, Ryuichi Tatsumi, Yoshihide Ikeuchi, Wataru Mizunoya

研究成果: ジャーナルへの寄稿記事

8 引用 (Scopus)

抄録

Skeletal muscle is the main tissue of lipid metabolism and accordingly is critical for homeostasis and energy production; however, the determinants of lipid accumulation in skeletal muscle are unknown. Here, we examined whether the soleus muscle (predominantly slow-twitch fibers) has a higher lipid accumulation capacity than that of the extensor digitorum longus (EDL, predominantly fast-twitch fibers) muscle in mice. Soleus and EDL muscles were harvested from male C57BL/6J mice. The mRNA levels of genes involved in fatty acid import and triglyceride synthesis and accumulation were examined in soleus and EDL muscles. The intramyocellular lipid (IMCL) droplets of muscle cross sections and isolated single fibers were visualized by staining with BODIPY493/503, and fiber types were determined by immunofluorescent detection of myosin heavy chain (MyHC) isoforms. We detected higher mRNA expression of genes related to lipid accumulation in the soleus than the EDL. We also observed a marked increase of IMCL in single fibers from the soleus, but not the EDL, after treatment with a high-fat diet plus denervation. Interestingly, greater accumulation of IMCL droplets was observed in type 2A and 2X fibers (MyHC2A- and MyHC2X-positive fibers) than type 1 fibers (MyHC1-positive fibers) in soleus muscles. These results suggest that the soleus contains more IMCL owing to the higher population of type 2A fibers, and the difference in lipid accumulation between the soleus and EDL could depend on fiber type composition.

元の言語英語
ページ(範囲)163-173
ページ数11
ジャーナルJournal of Muscle Research and Cell Motility
38
発行部数2
DOI
出版物ステータス出版済み - 4 1 2017

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Muscle
Skeletal Muscle
Lipids
Muscles
Fibers
Fast-Twitch Muscle Fibers
Messenger RNA
Myosin Heavy Chains
High Fat Diet
Denervation
Inbred C57BL Mouse
Lipid Metabolism
Lipid Droplets
Genes
Protein Isoforms
Triglycerides
Homeostasis
Fatty Acids
Staining and Labeling
Gene Expression

All Science Journal Classification (ASJC) codes

  • Physiology
  • Biochemistry
  • Cell Biology

これを引用

Mouse soleus (slow) muscle shows greater intramyocellular lipid droplet accumulation than EDL (fast) muscle : fiber type-specific analysis. / Komiya, Yusuke; Sawano, Shoko; Mashima, Daisuke; Ichitsubo, Riho; Nakamura, Mako; Tatsumi, Ryuichi; Ikeuchi, Yoshihide; Mizunoya, Wataru.

:: Journal of Muscle Research and Cell Motility, 巻 38, 番号 2, 01.04.2017, p. 163-173.

研究成果: ジャーナルへの寄稿記事

Komiya, Yusuke ; Sawano, Shoko ; Mashima, Daisuke ; Ichitsubo, Riho ; Nakamura, Mako ; Tatsumi, Ryuichi ; Ikeuchi, Yoshihide ; Mizunoya, Wataru. / Mouse soleus (slow) muscle shows greater intramyocellular lipid droplet accumulation than EDL (fast) muscle : fiber type-specific analysis. :: Journal of Muscle Research and Cell Motility. 2017 ; 巻 38, 番号 2. pp. 163-173.
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abstract = "Skeletal muscle is the main tissue of lipid metabolism and accordingly is critical for homeostasis and energy production; however, the determinants of lipid accumulation in skeletal muscle are unknown. Here, we examined whether the soleus muscle (predominantly slow-twitch fibers) has a higher lipid accumulation capacity than that of the extensor digitorum longus (EDL, predominantly fast-twitch fibers) muscle in mice. Soleus and EDL muscles were harvested from male C57BL/6J mice. The mRNA levels of genes involved in fatty acid import and triglyceride synthesis and accumulation were examined in soleus and EDL muscles. The intramyocellular lipid (IMCL) droplets of muscle cross sections and isolated single fibers were visualized by staining with BODIPY493/503, and fiber types were determined by immunofluorescent detection of myosin heavy chain (MyHC) isoforms. We detected higher mRNA expression of genes related to lipid accumulation in the soleus than the EDL. We also observed a marked increase of IMCL in single fibers from the soleus, but not the EDL, after treatment with a high-fat diet plus denervation. Interestingly, greater accumulation of IMCL droplets was observed in type 2A and 2X fibers (MyHC2A- and MyHC2X-positive fibers) than type 1 fibers (MyHC1-positive fibers) in soleus muscles. These results suggest that the soleus contains more IMCL owing to the higher population of type 2A fibers, and the difference in lipid accumulation between the soleus and EDL could depend on fiber type composition.",
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T2 - fiber type-specific analysis

AU - Komiya, Yusuke

AU - Sawano, Shoko

AU - Mashima, Daisuke

AU - Ichitsubo, Riho

AU - Nakamura, Mako

AU - Tatsumi, Ryuichi

AU - Ikeuchi, Yoshihide

AU - Mizunoya, Wataru

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