TY - JOUR
T1 - Mouse strain differences in Gurmarin-sensitivity of sweet taste responses are not associated with polymorphisms of the sweet receptor gene, Tas1r3
AU - Sanematsu, Keisuke
AU - Yasumatsu, Keiko
AU - Yoshida, Ryusuke
AU - Shigemura, Noriatsu
AU - Ninomiya, Yuzo
N1 - Funding Information:
We thank Dr R.F. Margolskee for his valuable comments and suggestions on the manuscripts and Dr T. Imoto for helping us to purify Gur from the plant Gymnema sylvestre. This work was supported by Grant-in-Aids 15209061(Y.N.) for Scientific Research from Japan Society for the Promotion of Science.
PY - 2005/7
Y1 - 2005/7
N2 - Gurmarin (Gur) is a peptide that selectively inhibits responses of the chorda tympani (CT) nerve to sweet compounds in rodents. In mice, the sweet-suppressing effect of Gur differs among strains. The inhibitory effect of Gur is clearly observed in C57BL/6 mice, but only slightly, if at all, in BALB/c mice. These two mouse strains possess different alleles of the sweet receptor gene, Sac(Tas1r3) (taster genotype for C57BL/6 and non-taster genotype for BALB/c mice), suggesting that polymorphisms in the gene may account for differential sensitivity to Gur. To investigate this possibility, we examined the effect of Gur in another Tas1r3 non-taster strain, 129X1/Sv mice. The results indicated that unlike non-taster BALB/c mice but similar to taster C57BL/6 mice, 129X1/Sv mice exhibited significant inhibition of CT responses to various sweet compounds by Gur. This suggests that the mouse strain difference in the Gur inhibition of sweet responses of the CT nerve may not be associated with polymorphisms of Tas1r3.
AB - Gurmarin (Gur) is a peptide that selectively inhibits responses of the chorda tympani (CT) nerve to sweet compounds in rodents. In mice, the sweet-suppressing effect of Gur differs among strains. The inhibitory effect of Gur is clearly observed in C57BL/6 mice, but only slightly, if at all, in BALB/c mice. These two mouse strains possess different alleles of the sweet receptor gene, Sac(Tas1r3) (taster genotype for C57BL/6 and non-taster genotype for BALB/c mice), suggesting that polymorphisms in the gene may account for differential sensitivity to Gur. To investigate this possibility, we examined the effect of Gur in another Tas1r3 non-taster strain, 129X1/Sv mice. The results indicated that unlike non-taster BALB/c mice but similar to taster C57BL/6 mice, 129X1/Sv mice exhibited significant inhibition of CT responses to various sweet compounds by Gur. This suggests that the mouse strain difference in the Gur inhibition of sweet responses of the CT nerve may not be associated with polymorphisms of Tas1r3.
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U2 - 10.1093/chemse/bji041
DO - 10.1093/chemse/bji041
M3 - Article
C2 - 15932937
AN - SCOPUS:23844478321
VL - 30
SP - 491
EP - 496
JO - Chemical Senses
JF - Chemical Senses
SN - 0379-864X
IS - 6
ER -