Mucilage strongly binds aluminum but does not prevent roots from aluminum injury in Zea mays

Xiao Feng Li, Jian Feng Ma, Syuntaro Hiradate, Hideaki Matsumoto

研究成果: ジャーナルへの寄稿学術誌査読

59 被引用数 (Scopus)

抄録

The possible role of mucilage in protecting roots from aluminum (Al) injury was investigated in Zea mays L. (cv. Golden Cross Bantam), focusing on binding of Al with mucilage and effects of mucilage on Al toxicity. Al was bound to mucilage after the treatment of roots with 10-50 μM Al for 1 h and 30 μm Al for 30, 60, 90 and 120 min. Using molecular sieve chromatography (Sephadex G-100), Al was co-eluted with a high molecular mass sugar and a low molecular mass sugar. The difficulty in desorbing Al from mucilage with organic acids confirmed the strong binding strength of Al by mucilage revealed by 27Al nuclear magnetic resonance (NMR). Al could not be desorbed completely by succinic, malic, oxalic and citric acid at a molar ratio of 1:1. It could only be completely removed by oxalic acid at a molar ratio of 20:1 (oxalate:Al). Bioassay experiments showed that cell viability and callose formation were unaffected by Al bound to mucilage. However, mucilage deprived roots had only 0.21-0.59 nmol apex-1 higher Al content than control roots after treatment with 30 μM Al for 1, 1.5 and 2 h. Moreover, inhibition of root elongation by 5 μM Al for 6, 12, 24 and 36 h was independent of the presence or absence of mucilage prior to the Al treatment. These results indicate that although mucilage affects the accumulation of Al by roots, it does not confer Al resistance to Z. mays root apices.

本文言語英語
ページ(範囲)152-160
ページ数9
ジャーナルPhysiologia Plantarum
108
2
DOI
出版ステータス出版済み - 2月 2000
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 生理学
  • 遺伝学
  • 植物科学
  • 細胞生物学

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