Multifunctional hydrolytic catalyses x. Catalytic hydrolysis of p-Nitrophenyl acetate by a polyethylenimine derivative containing hydroxamate (Zwitterionic) group

Yoshio Okahata, Toyoki Kunitake

研究成果: Contribution to journalArticle査読

4 被引用数 (Scopus)

抄録

A water-soluble polyethylenimine derivatives was prepared containing the zwittenionic hydroxamate group, 11 mol% dococyl side chain, and a tertiary amino group. This polymer hydrolyzes p-nitrophenyl esters very effeciently (30°C, 3 v/v% EtOHH2O), according to the Michaelis-Menten kinetics. The catalysis involves substrate binding (Km= (2-5) × 10-4M in the case of p-nitrophenyl acetate) due to hydrophobic interaction, and facile acylation and de-acylation at the hydroxamate site. The zwitterionic nature and the hydrophobic microenvironment contribute to the enchanced nucleophilicity to the hydroxamate group. It is suggested that the de-acylation process involves general-base catalysis by the tert-amino group. The overall catalytic effeciency for p-nitrophenyl acetate exceeded that of α-chymotrypsin by a factor of ca. 100 under a comparable condition. Polymers which do not contain the hydroxamate group or contain the imidazole group in its place were much less effective.

本文言語英語
ページ(範囲)163-175
ページ数13
ジャーナルJournal of Molecular Catalysis
6
3
DOI
出版ステータス出版済み - 9 1979

All Science Journal Classification (ASJC) codes

  • 工学(全般)

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