Multifunctional rna binding protein ostudor-sn in storage protein mrna transport and localization

Hong Li Chou, Li Tian, Toshihiro Kumamaru, Shigeki Hamada, Thomas W. Okita

研究成果: ジャーナルへの寄稿記事

5 引用 (Scopus)

抄録

The multifunctional RNA-binding protein Tudor-SN plays multiple roles in transcriptional and posttranscriptional processes due to its modular domain structure, consisting of four tandem Staphylococcus nuclease (SN)-like domains (4SN), followed by a carboxyl-terminal Tudor domain, followed by a fifth partial SN sequence (Tsn). In plants, it confers stress tolerance, is a component of stress granules and P-bodies, and may participate in stabilizing and localizing RNAs to specific subdomains of the cortical-endoplasmic reticulum in developing rice (Oryza sativa) endosperm. Here, we show that, in addition to the intact rice OsTudor-SN protein, the 4SN and Tsn modules exist as independent polypeptides, which collectively may coassemble to form a complex population of homodimer and heteroduplex species. The 4SN and Tsn modules exhibit different roles in RNA binding and as a protein scaffold for stress-associated proteins and RNA-binding proteins. Despite their distinct individual properties, mutations in both the 4SN and Tsn modules mislocalize storage protein mRNAs to the cortical endoplasmic reticulum. These results indicate that the two modular peptide regions of OsTudor-SN confer different cellular properties but cooperate in mRNA localization, a process linking its multiple functions in the nucleus and cytoplasm.

元の言語英語
ページ(範囲)1608-1623
ページ数16
ジャーナルPlant physiology
175
発行部数4
DOI
出版物ステータス出版済み - 12 1 2017

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RNA-Binding Proteins
protein transport
nucleases
Protein Transport
storage proteins
Staphylococcus
binding proteins
RNA-binding proteins
Endoplasmic Reticulum
endoplasmic reticulum
scaffolding proteins
RNA
rice
Messenger RNA
Endosperm
Peptides
Proteins
Heat-Shock Proteins
Protein Binding
stress tolerance

All Science Journal Classification (ASJC) codes

  • Physiology
  • Genetics
  • Plant Science

これを引用

Multifunctional rna binding protein ostudor-sn in storage protein mrna transport and localization. / Chou, Hong Li; Tian, Li; Kumamaru, Toshihiro; Hamada, Shigeki; Okita, Thomas W.

:: Plant physiology, 巻 175, 番号 4, 01.12.2017, p. 1608-1623.

研究成果: ジャーナルへの寄稿記事

Chou, Hong Li ; Tian, Li ; Kumamaru, Toshihiro ; Hamada, Shigeki ; Okita, Thomas W. / Multifunctional rna binding protein ostudor-sn in storage protein mrna transport and localization. :: Plant physiology. 2017 ; 巻 175, 番号 4. pp. 1608-1623.
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