Multiphase-laden gas-liquid interface injection for the versatile gene transfer

H. Kuriki, S. Takasawa, M. Iwabuchi, K. Ohsumi, T. Suzuki, T. Higashiyama, S. Sakuma, F. Arai, Yoko Yamanishi

研究成果: 著書/レポートタイプへの貢献会議での発言

2 引用 (Scopus)

抄録

We have successfully carried out gene transfer using electrically induced bubble knife. The air-liquid interfaces of the dispensed bubbles have novel characteristics which can carry the various kinds of the materials in different phase and viscosity of solution. This technique has advantages in high efficiency of injection of limited amount of valuable samples such as designed m-RNA. In this paper, we demonstrated m-RNA encoding 3xFLAG-GST into xenopus oocyte to be sure that electrically induced bubble knife has ability to injection chemically unstable and biocompatibility. Furthermore, we injected fluorescent beads whose size was 2.1 μm into BY-2 cell as multiphase injection.

元の言語英語
ホスト出版物のタイトル17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
出版者Chemical and Biological Microsystems Society
ページ1173-1175
ページ数3
ISBN(印刷物)9781632666246
出版物ステータス出版済み - 1 1 2013
イベント17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, ドイツ
継続期間: 10 27 201310 31 2013

出版物シリーズ

名前17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
2

その他

その他17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
ドイツ
Freiburg
期間10/27/1310/31/13

Fingerprint

Gene transfer
RNA
Bubbles (in fluids)
Gases
Liquids
Biocompatibility
Viscosity
Air

All Science Journal Classification (ASJC) codes

  • Bioengineering

これを引用

Kuriki, H., Takasawa, S., Iwabuchi, M., Ohsumi, K., Suzuki, T., Higashiyama, T., ... Yamanishi, Y. (2013). Multiphase-laden gas-liquid interface injection for the versatile gene transfer. : 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 (pp. 1173-1175). (17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; 巻数 2). Chemical and Biological Microsystems Society.

Multiphase-laden gas-liquid interface injection for the versatile gene transfer. / Kuriki, H.; Takasawa, S.; Iwabuchi, M.; Ohsumi, K.; Suzuki, T.; Higashiyama, T.; Sakuma, S.; Arai, F.; Yamanishi, Yoko.

17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Chemical and Biological Microsystems Society, 2013. p. 1173-1175 (17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; 巻 2).

研究成果: 著書/レポートタイプへの貢献会議での発言

Kuriki, H, Takasawa, S, Iwabuchi, M, Ohsumi, K, Suzuki, T, Higashiyama, T, Sakuma, S, Arai, F & Yamanishi, Y 2013, Multiphase-laden gas-liquid interface injection for the versatile gene transfer. : 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, 巻. 2, Chemical and Biological Microsystems Society, pp. 1173-1175, 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, Freiburg, ドイツ, 10/27/13.
Kuriki H, Takasawa S, Iwabuchi M, Ohsumi K, Suzuki T, Higashiyama T その他. Multiphase-laden gas-liquid interface injection for the versatile gene transfer. : 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Chemical and Biological Microsystems Society. 2013. p. 1173-1175. (17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013).
Kuriki, H. ; Takasawa, S. ; Iwabuchi, M. ; Ohsumi, K. ; Suzuki, T. ; Higashiyama, T. ; Sakuma, S. ; Arai, F. ; Yamanishi, Yoko. / Multiphase-laden gas-liquid interface injection for the versatile gene transfer. 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Chemical and Biological Microsystems Society, 2013. pp. 1173-1175 (17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013).
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