Mutant mouse lysozyme carrying a minimal T cell epitope of hen egg lysozyme evokes high autoantibody response

Y. Tsujihata, T. So, Y. Chijiiwa, Y. Hashimoto, M. Hirata, T. Ueda, T. Imoto

研究成果: ジャーナルへの寄稿記事

10 引用 (Scopus)

抄録

Self proteins including foreign T cell epitope induce autoantibodies. We evaluated the relationship between the size of foreign Ag introduced into self protein and the magnitude of autoantibody production. Mouse lysozyme (ML) was used as a model self protein, and we prepared three different ML derivatives carrying T cell epitope of hen egg white lysozyme (HEL) 107-116, i.e., heterodimer of ML and HEL (ML-HEL), chimeric lysozyme that has residue 1-82 of ML and residue 83-130 of HEL in its sequence (chiMH), and mutant ML that has triple mutations rendering the most potent T cell epitope of HEL (sequence 107-116). Immunization of BALB/c mice with these three ML derivatives induced anti-ML autoantibody responses, whereas native ML induced no detectable response. In particular, mutML generated a 104 times higher autoanfibody titer than did ML-HEL. Anti-HEL107-116 T cell-priming activities were almost similar among the ML derivatives. The heterodimerization of mutant ML and HEL led to significant reduction of the autoantibody response, whereas the mixture did not. These results show that size of the nonself region in modified self Ag has an important role in determining the magnitude of the autoantibody response, and that decrease in the foreign region in a modified self protein may cause high-titered autoantibody response.

元の言語英語
ページ(範囲)3606-3611
ページ数6
ジャーナルJournal of Immunology
165
発行部数7
DOI
出版物ステータス出版済み - 10 1 2000

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T-Lymphocyte Epitopes
Muramidase
Autoantibodies
Egg White
hen egg lysozyme
Proteins
Immunization

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

これを引用

Mutant mouse lysozyme carrying a minimal T cell epitope of hen egg lysozyme evokes high autoantibody response. / Tsujihata, Y.; So, T.; Chijiiwa, Y.; Hashimoto, Y.; Hirata, M.; Ueda, T.; Imoto, T.

:: Journal of Immunology, 巻 165, 番号 7, 01.10.2000, p. 3606-3611.

研究成果: ジャーナルへの寄稿記事

Tsujihata, Y. ; So, T. ; Chijiiwa, Y. ; Hashimoto, Y. ; Hirata, M. ; Ueda, T. ; Imoto, T. / Mutant mouse lysozyme carrying a minimal T cell epitope of hen egg lysozyme evokes high autoantibody response. :: Journal of Immunology. 2000 ; 巻 165, 番号 7. pp. 3606-3611.
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abstract = "Self proteins including foreign T cell epitope induce autoantibodies. We evaluated the relationship between the size of foreign Ag introduced into self protein and the magnitude of autoantibody production. Mouse lysozyme (ML) was used as a model self protein, and we prepared three different ML derivatives carrying T cell epitope of hen egg white lysozyme (HEL) 107-116, i.e., heterodimer of ML and HEL (ML-HEL), chimeric lysozyme that has residue 1-82 of ML and residue 83-130 of HEL in its sequence (chiMH), and mutant ML that has triple mutations rendering the most potent T cell epitope of HEL (sequence 107-116). Immunization of BALB/c mice with these three ML derivatives induced anti-ML autoantibody responses, whereas native ML induced no detectable response. In particular, mutML generated a 104 times higher autoanfibody titer than did ML-HEL. Anti-HEL107-116 T cell-priming activities were almost similar among the ML derivatives. The heterodimerization of mutant ML and HEL led to significant reduction of the autoantibody response, whereas the mixture did not. These results show that size of the nonself region in modified self Ag has an important role in determining the magnitude of the autoantibody response, and that decrease in the foreign region in a modified self protein may cause high-titered autoantibody response.",
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AU - So, T.

AU - Chijiiwa, Y.

AU - Hashimoto, Y.

AU - Hirata, M.

AU - Ueda, T.

AU - Imoto, T.

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