Mutants for rice storage proteins - 1. Screening of mutants for rice storage proteins of protein bodies in the starchy endosperm

Toshihiro Kumamaru, H. Satoh, N. Iwata, T. Omura, M. Ogawa, K. Tanaka

研究成果: ジャーナルへの寄稿記事

67 引用 (Scopus)

抄録

To obtain genetic materials to breed qualitatively improved rice storage proteins, we screened about 3,000 mutant lines induced by the treatment of rice fertilized egg cell with N-methyl-N-nitrosourea (MNU). The screening was performed by comparing the profiles of sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) with that of the original variety, Kinmaze, especially focussing on the changes in polypeptides present in two kinds of protein bodies, PB-I and PB-II. We selected 17 mutant lines and classified them into 4 types on the basis of variations of the relative contents of the polypeptides. Determination of extracted protein in the starchy endosperm of the mutants revealed changes in the content of prolamin and glutelin but not globulin. In some mutants there was marked accumulation of 57 kDa polypeptide concomitant with the remarkable reduction of glutelin subunits. Treatment of the fertilized egg cell with MNU was found to be an effective method to induce mutations for storage proteins in protein bodies of rice.

元の言語英語
ページ(範囲)11-16
ページ数6
ジャーナルTheoretical And Applied Genetics
76
発行部数1
DOI
出版物ステータス出版済み - 7 1 1988

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rice protein
Endosperm
protein bodies
storage proteins
endosperm
screening
polypeptides
mutants
glutelins
ova
Glutens
Zygote
Proteins
N-methyl-N-nitrosourea
Peptides
rice
prolamins
Prolamins
Methylnitrosourea
globulins

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science
  • Genetics

これを引用

Mutants for rice storage proteins - 1. Screening of mutants for rice storage proteins of protein bodies in the starchy endosperm. / Kumamaru, Toshihiro; Satoh, H.; Iwata, N.; Omura, T.; Ogawa, M.; Tanaka, K.

:: Theoretical And Applied Genetics, 巻 76, 番号 1, 01.07.1988, p. 11-16.

研究成果: ジャーナルへの寄稿記事

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AU - Ogawa, M.

AU - Tanaka, K.

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