Mutants for rice storage proteins - 2. Isolation and characterization of protein bodies from rice mutants

M. Ogawa, Toshihiro Kumamaru, H. Satoh, T. Omura, T. Park, K. Shintaku, K. Baba

研究成果: ジャーナルへの寄稿記事

23 引用 (Scopus)

抄録

Rice storage proteins of the endosperm are localized in two types of protein bodies, PB-I and PB-II. Protein bodies were isolated by sucrose density gradient centrifugation from developing endosperm of three rice mutants, CM 21, CM 1675 and CM 1834, and characterized after pepsin-digestion treatment by protein contents determination. Mutant protein bodies (PBs) except for their internal structure, were similar in shape and density to PB-I of the variety Kinmaze. Electrophoretic analysis of PB-I polypeptides revealed that SDS (Sodium dodecylsulfate) bands of 13 and 16 kilodaltons consisted, respectively, of four and two individual polypeptides with different pI values, while the 10-kilodalton band behaved as a single polypeptide after isoelectric focussing (IEF) electrophoresis. The differences in the polypeptide composition induced by mutants were due to the decrease and/or increase in the content of specific PB-I polypeptides. Electron microscopic observations revealed that the typical lamellar structure of the PB-I is not visible in CM 1675. On the contrary, the inner portion of PB-I in CM 1834 and CM 21 showed higher electron density than that of the variety Kinmaze. On these two mutants, the content of pepsin-indigestible and -digestible proteins were similar to those of Kinmaze, although the values of the PB-II/PB-I ratio were greater than those for Kinmaze, suggesting that these two mutants are high-glutelin rice mutants.

元の言語英語
ページ(範囲)305-310
ページ数6
ジャーナルTheoretical And Applied Genetics
78
発行部数3
DOI
出版物ステータス出版済み - 9 1 1989
外部発表Yes

Fingerprint

rice protein
protein bodies
storage proteins
rice
mutants
Proteins
polypeptides
Peptides
Endosperm
Pepsin A
pepsin
endosperm
Oryza
electrons
Electrons
glutelins
Somatotypes
body protein
Density Gradient Centrifugation
Glutens

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science
  • Genetics

これを引用

Mutants for rice storage proteins - 2. Isolation and characterization of protein bodies from rice mutants. / Ogawa, M.; Kumamaru, Toshihiro; Satoh, H.; Omura, T.; Park, T.; Shintaku, K.; Baba, K.

:: Theoretical And Applied Genetics, 巻 78, 番号 3, 01.09.1989, p. 305-310.

研究成果: ジャーナルへの寄稿記事

Ogawa, M. ; Kumamaru, Toshihiro ; Satoh, H. ; Omura, T. ; Park, T. ; Shintaku, K. ; Baba, K. / Mutants for rice storage proteins - 2. Isolation and characterization of protein bodies from rice mutants. :: Theoretical And Applied Genetics. 1989 ; 巻 78, 番号 3. pp. 305-310.
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abstract = "Rice storage proteins of the endosperm are localized in two types of protein bodies, PB-I and PB-II. Protein bodies were isolated by sucrose density gradient centrifugation from developing endosperm of three rice mutants, CM 21, CM 1675 and CM 1834, and characterized after pepsin-digestion treatment by protein contents determination. Mutant protein bodies (PBs) except for their internal structure, were similar in shape and density to PB-I of the variety Kinmaze. Electrophoretic analysis of PB-I polypeptides revealed that SDS (Sodium dodecylsulfate) bands of 13 and 16 kilodaltons consisted, respectively, of four and two individual polypeptides with different pI values, while the 10-kilodalton band behaved as a single polypeptide after isoelectric focussing (IEF) electrophoresis. The differences in the polypeptide composition induced by mutants were due to the decrease and/or increase in the content of specific PB-I polypeptides. Electron microscopic observations revealed that the typical lamellar structure of the PB-I is not visible in CM 1675. On the contrary, the inner portion of PB-I in CM 1834 and CM 21 showed higher electron density than that of the variety Kinmaze. On these two mutants, the content of pepsin-indigestible and -digestible proteins were similar to those of Kinmaze, although the values of the PB-II/PB-I ratio were greater than those for Kinmaze, suggesting that these two mutants are high-glutelin rice mutants.",
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