Mutation analysis utilizing DNA intercalation in reversed micelles

Park Lianchun, Miki Funai, Masahiro Goto

    研究成果: Contribution to journalArticle査読

    抄録

    A new mutation analysis system was developed by utilizing a DNA intercalator in reversed micelles. The hybridization signal was amplified by monitoring the intercalation behavior between a porphirine derivative and a double strand DNA in reversed micelles. Hybridization rate in reversed micelles was analyzed for three sample DNAs bearing a mutation in a different part. The location of the mutation significantly affected the hybridization behavior. This novel method allows the DNA concentration to be reduced to less than one-tenth of that employed in the conventional reversed micellar method.

    本文言語英語
    ページ(範囲)776-778
    ページ数3
    ジャーナルkagaku kogaku ronbunshu
    28
    6
    DOI
    出版ステータス出版済み - 11 2002

    All Science Journal Classification (ASJC) codes

    • 化学 (全般)
    • 化学工学(全般)

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