Mutation analysis utilizing DNA intercalation in reversed micelles

Park Lianchun, Miki Funai, Masahiro Goto

研究成果: Contribution to journalArticle

抜粋

A new mutation analysis system was developed by utilizing a DNA intercalator in reversed micelles. The hybridization signal was amplified by monitoring the intercalation behavior between a porphirine derivative and a double strand DNA in reversed micelles. Hybridization rate in reversed micelles was analyzed for three sample DNAs bearing a mutation in a different part. The location of the mutation significantly affected the hybridization behavior. This novel method allows the DNA concentration to be reduced to less than one-tenth of that employed in the conventional reversed micellar method.

元の言語英語
ページ(範囲)776-778
ページ数3
ジャーナルKagaku Kogaku Ronbunshu
28
発行部数6
DOI
出版物ステータス出版済み - 1 1 2002

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Chemical Engineering(all)

フィンガープリント Mutation analysis utilizing DNA intercalation in reversed micelles' の研究トピックを掘り下げます。これらはともに一意のフィンガープリントを構成します。

  • これを引用