Mutation in fission yeast phosphatidylinositol 4-kinase Pik1 is synthetically lethal with defect in telomere protection protein Pot1

Asami Sugihara, Luan Cao Nguyen, Hossain Mohammad Shamim, Tetsushi Iida, Mai Nakase, Kaoru Takegawa, Mitsuhisa Senda, Shohei Jida, Masaru Ueno

研究成果: ジャーナルへの寄稿記事

抄録

Fission yeast Pik1p is one of three phosphatidylinositol 4-kinases associated with the Golgi complex, but its function is not fully understood. Deletion of pot1+ causes telomere degradation and chromosome circularization. We searched for the gene which becomes synthetically lethal with pot1Δ. We obtained a novel pik1 mutant, pik1-1, which is synthetically lethal with pot1Δ. We found phosphoinositol 4-phosphate in the Golgi was reduced in pik1-1. To investigate the mechanism of the lethality of the pot1Δ pik1-1 double mutant, we constructed the nmt-pot1-aid pik1-1 strain, where Pot1 function becomes low by drugs, which leads to telomere loss and chromosome circularization, and found pik1-1 mutation does not affect telomere resection and chromosome circularization. Thus, our results suggest that pik1+ is required for the maintenance of circular chromosomes.

元の言語英語
ページ(範囲)1284-1290
ページ数7
ジャーナルBiochemical and Biophysical Research Communications
496
発行部数4
DOI
出版物ステータス出版済み - 2 19 2018

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1-Phosphatidylinositol 4-Kinase
Schizosaccharomyces
Telomere
Chromosomes
Yeast
Defects
Mutation
Proteins
Golgi Apparatus
Genes
Phosphates
Maintenance
Degradation
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

これを引用

Mutation in fission yeast phosphatidylinositol 4-kinase Pik1 is synthetically lethal with defect in telomere protection protein Pot1. / Sugihara, Asami; Nguyen, Luan Cao; Shamim, Hossain Mohammad; Iida, Tetsushi; Nakase, Mai; Takegawa, Kaoru; Senda, Mitsuhisa; Jida, Shohei; Ueno, Masaru.

:: Biochemical and Biophysical Research Communications, 巻 496, 番号 4, 19.02.2018, p. 1284-1290.

研究成果: ジャーナルへの寄稿記事

Sugihara, Asami ; Nguyen, Luan Cao ; Shamim, Hossain Mohammad ; Iida, Tetsushi ; Nakase, Mai ; Takegawa, Kaoru ; Senda, Mitsuhisa ; Jida, Shohei ; Ueno, Masaru. / Mutation in fission yeast phosphatidylinositol 4-kinase Pik1 is synthetically lethal with defect in telomere protection protein Pot1. :: Biochemical and Biophysical Research Communications. 2018 ; 巻 496, 番号 4. pp. 1284-1290.
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abstract = "Fission yeast Pik1p is one of three phosphatidylinositol 4-kinases associated with the Golgi complex, but its function is not fully understood. Deletion of pot1+ causes telomere degradation and chromosome circularization. We searched for the gene which becomes synthetically lethal with pot1Δ. We obtained a novel pik1 mutant, pik1-1, which is synthetically lethal with pot1Δ. We found phosphoinositol 4-phosphate in the Golgi was reduced in pik1-1. To investigate the mechanism of the lethality of the pot1Δ pik1-1 double mutant, we constructed the nmt-pot1-aid pik1-1 strain, where Pot1 function becomes low by drugs, which leads to telomere loss and chromosome circularization, and found pik1-1 mutation does not affect telomere resection and chromosome circularization. Thus, our results suggest that pik1+ is required for the maintenance of circular chromosomes.",
author = "Asami Sugihara and Nguyen, {Luan Cao} and Shamim, {Hossain Mohammad} and Tetsushi Iida and Mai Nakase and Kaoru Takegawa and Mitsuhisa Senda and Shohei Jida and Masaru Ueno",
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AU - Sugihara, Asami

AU - Nguyen, Luan Cao

AU - Shamim, Hossain Mohammad

AU - Iida, Tetsushi

AU - Nakase, Mai

AU - Takegawa, Kaoru

AU - Senda, Mitsuhisa

AU - Jida, Shohei

AU - Ueno, Masaru

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Y1 - 2018/2/19

N2 - Fission yeast Pik1p is one of three phosphatidylinositol 4-kinases associated with the Golgi complex, but its function is not fully understood. Deletion of pot1+ causes telomere degradation and chromosome circularization. We searched for the gene which becomes synthetically lethal with pot1Δ. We obtained a novel pik1 mutant, pik1-1, which is synthetically lethal with pot1Δ. We found phosphoinositol 4-phosphate in the Golgi was reduced in pik1-1. To investigate the mechanism of the lethality of the pot1Δ pik1-1 double mutant, we constructed the nmt-pot1-aid pik1-1 strain, where Pot1 function becomes low by drugs, which leads to telomere loss and chromosome circularization, and found pik1-1 mutation does not affect telomere resection and chromosome circularization. Thus, our results suggest that pik1+ is required for the maintenance of circular chromosomes.

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