Mutations in an amino acid transporter gene are responsible for sex-linked translucent larval skin of the silkworm, Bombyx mori

Takashi Kiuchi, Yutaka Banno, Susumu Katsuma, Toru Shimada

研究成果: ジャーナルへの寄稿記事

15 引用 (Scopus)

抄録

The sex-linked translucent (os) mutation in the silkworm, Bombyx mori, confers slightly translucent larval skin resulting from a decrease in the incorporation of uric acid into epidermal cells. By positional cloning, we narrowed a region linked to the os phenotype to approximately 157 kb located on scaffold Bm_scaf72 on the Z chromosome (chromosome 1). The region contained four gene models. Sequencing analysis revealed that one of the candidate genes had a 7-bp deletion in the coding region. We also found a 111-bp deletion or single-nucleotide substitution in the same gene using independent os mutant strains. Because all the mutations caused the generation of abnormal transcripts followed by translation of a truncated protein, we conclude that the mutation of this candidate gene is responsible for the translucent larval skin of the os mutant. Sequence analysis indicated that the gene responsible for the os mutation had homology to amino acid transporters of the solute carrier family of proteins. Our results suggest that solute carrier proteins are involved in uric acid transport in insects and other invertebrates.

元の言語英語
ページ(範囲)680-687
ページ数8
ジャーナルInsect Biochemistry and Molecular Biology
41
発行部数9
DOI
出版物ステータス出版済み - 9 1 2011

Fingerprint

amino acid transporters
Safe Sex
Amino Acid Transport Systems
Bombyx
Bombyx mori
silkworms
skin (animal)
Skin
Genes
mutation
Mutation
gender
uric acid
Chromosomes
Uric Acid
genes
solutes
Z chromosome
mutants
Cloning

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Insect Science

これを引用

Mutations in an amino acid transporter gene are responsible for sex-linked translucent larval skin of the silkworm, Bombyx mori. / Kiuchi, Takashi; Banno, Yutaka; Katsuma, Susumu; Shimada, Toru.

:: Insect Biochemistry and Molecular Biology, 巻 41, 番号 9, 01.09.2011, p. 680-687.

研究成果: ジャーナルへの寄稿記事

@article{b10765f42e544cc89eedee15854b342b,
title = "Mutations in an amino acid transporter gene are responsible for sex-linked translucent larval skin of the silkworm, Bombyx mori",
abstract = "The sex-linked translucent (os) mutation in the silkworm, Bombyx mori, confers slightly translucent larval skin resulting from a decrease in the incorporation of uric acid into epidermal cells. By positional cloning, we narrowed a region linked to the os phenotype to approximately 157 kb located on scaffold Bm_scaf72 on the Z chromosome (chromosome 1). The region contained four gene models. Sequencing analysis revealed that one of the candidate genes had a 7-bp deletion in the coding region. We also found a 111-bp deletion or single-nucleotide substitution in the same gene using independent os mutant strains. Because all the mutations caused the generation of abnormal transcripts followed by translation of a truncated protein, we conclude that the mutation of this candidate gene is responsible for the translucent larval skin of the os mutant. Sequence analysis indicated that the gene responsible for the os mutation had homology to amino acid transporters of the solute carrier family of proteins. Our results suggest that solute carrier proteins are involved in uric acid transport in insects and other invertebrates.",
author = "Takashi Kiuchi and Yutaka Banno and Susumu Katsuma and Toru Shimada",
year = "2011",
month = "9",
day = "1",
doi = "10.1016/j.ibmb.2011.04.011",
language = "English",
volume = "41",
pages = "680--687",
journal = "Insect Biochemistry and Molecular Biology",
issn = "0965-1748",
publisher = "Elsevier Limited",
number = "9",

}

TY - JOUR

T1 - Mutations in an amino acid transporter gene are responsible for sex-linked translucent larval skin of the silkworm, Bombyx mori

AU - Kiuchi, Takashi

AU - Banno, Yutaka

AU - Katsuma, Susumu

AU - Shimada, Toru

PY - 2011/9/1

Y1 - 2011/9/1

N2 - The sex-linked translucent (os) mutation in the silkworm, Bombyx mori, confers slightly translucent larval skin resulting from a decrease in the incorporation of uric acid into epidermal cells. By positional cloning, we narrowed a region linked to the os phenotype to approximately 157 kb located on scaffold Bm_scaf72 on the Z chromosome (chromosome 1). The region contained four gene models. Sequencing analysis revealed that one of the candidate genes had a 7-bp deletion in the coding region. We also found a 111-bp deletion or single-nucleotide substitution in the same gene using independent os mutant strains. Because all the mutations caused the generation of abnormal transcripts followed by translation of a truncated protein, we conclude that the mutation of this candidate gene is responsible for the translucent larval skin of the os mutant. Sequence analysis indicated that the gene responsible for the os mutation had homology to amino acid transporters of the solute carrier family of proteins. Our results suggest that solute carrier proteins are involved in uric acid transport in insects and other invertebrates.

AB - The sex-linked translucent (os) mutation in the silkworm, Bombyx mori, confers slightly translucent larval skin resulting from a decrease in the incorporation of uric acid into epidermal cells. By positional cloning, we narrowed a region linked to the os phenotype to approximately 157 kb located on scaffold Bm_scaf72 on the Z chromosome (chromosome 1). The region contained four gene models. Sequencing analysis revealed that one of the candidate genes had a 7-bp deletion in the coding region. We also found a 111-bp deletion or single-nucleotide substitution in the same gene using independent os mutant strains. Because all the mutations caused the generation of abnormal transcripts followed by translation of a truncated protein, we conclude that the mutation of this candidate gene is responsible for the translucent larval skin of the os mutant. Sequence analysis indicated that the gene responsible for the os mutation had homology to amino acid transporters of the solute carrier family of proteins. Our results suggest that solute carrier proteins are involved in uric acid transport in insects and other invertebrates.

UR - http://www.scopus.com/inward/record.url?scp=79959809185&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79959809185&partnerID=8YFLogxK

U2 - 10.1016/j.ibmb.2011.04.011

DO - 10.1016/j.ibmb.2011.04.011

M3 - Article

C2 - 21619931

AN - SCOPUS:79959809185

VL - 41

SP - 680

EP - 687

JO - Insect Biochemistry and Molecular Biology

JF - Insect Biochemistry and Molecular Biology

SN - 0965-1748

IS - 9

ER -