Mutations responsible for alcohol tolerance in the mutant of Synechococcus elongatus PCC 7942 (SY1043) obtained by single-cell screening system

Yasutaka Hirokawa, Yu Kanesaki, Sayuri Arai, Fumiko Saruta, Kayoko Hayashihara, Akio Murakami, Kazunori Shimizu, Hiroyuki Honda, Hirofumi Yoshikawa, Taizo Hanai

研究成果: ジャーナルへの寄稿記事

2 引用 (Scopus)

抄録

The production of alcohols directly from carbon dioxide by engineered cyanobacteria is an attractive technology for a sustainable future. Enhanced tolerance to the produced alcohols would be a desirable feature of the engineered cyanobacterial strains with higher alcohol productivity. We have recently obtained the mutant strains of Synechococcus elongatus PCC 7942 with higher tolerance to isopropanol using a single-cell screening system (Arai et al., Biotechnol. Bioeng., 114, 1771–1778, 2017). Among the mutant strains, SY1043 showed the highest isopropanol tolerance. Interestingly, SY1043 also showed higher tolerance to other alcohols such as ethanol and 1-butanol, however, the mechanisms involved in enhancing this alcohol tolerance were unclear. To reveal the alcohol tolerance mechanism of SY1043, we investigated the relationship between alcohol tolerance and four mutations found in SY1043 by genome resequencing analysis. Isopropanol tolerance was enhanced by amino acid substitution (Leu285Pro) in a hypothetical protein encoded by Synpcc7942_0180 of the wild type strain TA1297. TA4135, into which this mutation was introduced, showed a same tendency of tolerance to other alcohols (ethanol and 1-butanol).

元の言語英語
ページ(範囲)572-577
ページ数6
ジャーナルJournal of Bioscience and Bioengineering
125
発行部数5
DOI
出版物ステータス出版済み - 5 1 2018

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Synechococcus
Screening
Alcohols
Mutation
2-Propanol
1-Butanol
Butenes
Ethanol
Cyanobacteria
Amino Acid Substitution
Carbon Dioxide
Amino acids
Carbon dioxide
Genome
Substitution reactions
Genes
Productivity
Technology
Proteins
Amino Acids

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

これを引用

Mutations responsible for alcohol tolerance in the mutant of Synechococcus elongatus PCC 7942 (SY1043) obtained by single-cell screening system. / Hirokawa, Yasutaka; Kanesaki, Yu; Arai, Sayuri; Saruta, Fumiko; Hayashihara, Kayoko; Murakami, Akio; Shimizu, Kazunori; Honda, Hiroyuki; Yoshikawa, Hirofumi; Hanai, Taizo.

:: Journal of Bioscience and Bioengineering, 巻 125, 番号 5, 01.05.2018, p. 572-577.

研究成果: ジャーナルへの寄稿記事

Hirokawa, Yasutaka ; Kanesaki, Yu ; Arai, Sayuri ; Saruta, Fumiko ; Hayashihara, Kayoko ; Murakami, Akio ; Shimizu, Kazunori ; Honda, Hiroyuki ; Yoshikawa, Hirofumi ; Hanai, Taizo. / Mutations responsible for alcohol tolerance in the mutant of Synechococcus elongatus PCC 7942 (SY1043) obtained by single-cell screening system. :: Journal of Bioscience and Bioengineering. 2018 ; 巻 125, 番号 5. pp. 572-577.
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AU - Hirokawa, Yasutaka

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AU - Arai, Sayuri

AU - Saruta, Fumiko

AU - Hayashihara, Kayoko

AU - Murakami, Akio

AU - Shimizu, Kazunori

AU - Honda, Hiroyuki

AU - Yoshikawa, Hirofumi

AU - Hanai, Taizo

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