Mutual Regulation of Protein-tyrosine Phosphatase 20 and Protein-tyrosine Kinase Tec Activities by Tyrosine Phosphorylation and Dephosphorylation

Naohito Aokit, Shuichi Ueno, Hiroyuki Mano, Sho Yamasaki, Masayuki Shiota, Hitoshi Miyazaki, Yumiko Yamaguchi-Aoki, Tsukasa Matsuda, Axel Ullrich

    研究成果: Contribution to journalArticle査読

    18 被引用数 (Scopus)

    抄録

    PTP20, also known as HSCF/protein-tyrosine phosphatase K1/fetal liver phosphatase 1/brain-derived phosphatase 1, is a cytosolic protein-tyrosine phosphatase with currently unknown biological relevance. We have identified that the nonreceptor protein-tyrosine kinase Tec-phosphorylated PTP20 on tyrosines and co-immunoprecipitated with the phosphatase in a phosphotyrosine-dependent manner. The interaction between the two proteins involved the Tec SH2 domain and the C-terminal tyrosine residues Tyr-281, Tyr-303, Tyr-354, and Tyr-381 of PTP20, which were also necessary for tyrosine phosphorylation/dephosphorylation. Association between endogenous PTP20 and Tec was also tyrosine phosphorylation-dependent in the immature B cell line Ramos. Finally, the Tyr-281 residue of PTP20 was shown to be critical for deactivating Tec in Ramos cells upon B cell receptor ligation as well as dephosphorylation and deactivation of Tec and PTP20 itself in transfected COS7 cells. Taken together, PTP20 appears to play a negative role in Tec-mediated signaling, and Tec-PTP20 interaction might represent a negative feedback mechanism.

    本文言語英語
    ページ(範囲)10765-10775
    ページ数11
    ジャーナルJournal of Biological Chemistry
    279
    11
    DOI
    出版ステータス出版済み - 3 12 2004

    All Science Journal Classification (ASJC) codes

    • 生化学
    • 分子生物学
    • 細胞生物学

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