MyoD targets chromatin remodeling complexes to the myogenin locus prior to forming a stable DNA-bound complex

Ivana L. De La Serna, Yasuyuki Ohkawa, Charlotte A. Berkes, Donald A. Bergstrom, Caroline S. Dacwag, Stephen J. Tapscott, Anthony N. Imbalzano

研究成果: ジャーナルへの寄稿記事

191 引用 (Scopus)

抄録

The activation of muscle-specific gene expression requires the coordinated action of muscle regulatory proteins and chromatin-remodeling enzymes. Microarray analysis performed in the presence or absence of a dominant-negative BRG1 ATPase demonstrated that approximately one-third of MyoD-induced genes were highly dependent on SWI/SNF enzymes. To understand the mechanism of activation, we performed chromatin immunoprecipitations analyzing the myogenin promoter. We found that H4 hyperacetylation preceded Brg1 binding in a MyoD-dependent manner but that MyoD binding occurred subsequent to H4 modification and Brg1 interaction. In the absence of functional SWI/SNF enzymes, muscle regulatory proteins did not bind to the myogenin promoter, thereby providing evidence for SWI/SNF-dependent activator binding. We observed that the homeodomain factor Pbx1, which cooperates with MyoD to stimulate myogenin expression, is constitutively bound to the myogenin promoter in a SWI/SNF-independent manner, suggesting a two-step mechanism in which MyoD initially interacts indirectly with the myogenin promoter and attracts chromatin-remodeling enzymes, which then facilitate direct binding by MyoD and other regulatory proteins.

元の言語英語
ページ(範囲)3997-4009
ページ数13
ジャーナルMolecular and cellular biology
25
発行部数10
DOI
出版物ステータス出版済み - 5 1 2005

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Myogenin
Chromatin Assembly and Disassembly
DNA
Muscle Proteins
Enzymes
Chromatin Immunoprecipitation
Microarray Analysis
Adenosine Triphosphatases
Gene Expression
Muscles
Genes
Proteins

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

これを引用

De La Serna, I. L., Ohkawa, Y., Berkes, C. A., Bergstrom, D. A., Dacwag, C. S., Tapscott, S. J., & Imbalzano, A. N. (2005). MyoD targets chromatin remodeling complexes to the myogenin locus prior to forming a stable DNA-bound complex. Molecular and cellular biology, 25(10), 3997-4009. https://doi.org/10.1128/MCB.25.10.3997-4009.2005

MyoD targets chromatin remodeling complexes to the myogenin locus prior to forming a stable DNA-bound complex. / De La Serna, Ivana L.; Ohkawa, Yasuyuki; Berkes, Charlotte A.; Bergstrom, Donald A.; Dacwag, Caroline S.; Tapscott, Stephen J.; Imbalzano, Anthony N.

:: Molecular and cellular biology, 巻 25, 番号 10, 01.05.2005, p. 3997-4009.

研究成果: ジャーナルへの寄稿記事

De La Serna, IL, Ohkawa, Y, Berkes, CA, Bergstrom, DA, Dacwag, CS, Tapscott, SJ & Imbalzano, AN 2005, 'MyoD targets chromatin remodeling complexes to the myogenin locus prior to forming a stable DNA-bound complex', Molecular and cellular biology, 巻. 25, 番号 10, pp. 3997-4009. https://doi.org/10.1128/MCB.25.10.3997-4009.2005
De La Serna, Ivana L. ; Ohkawa, Yasuyuki ; Berkes, Charlotte A. ; Bergstrom, Donald A. ; Dacwag, Caroline S. ; Tapscott, Stephen J. ; Imbalzano, Anthony N. / MyoD targets chromatin remodeling complexes to the myogenin locus prior to forming a stable DNA-bound complex. :: Molecular and cellular biology. 2005 ; 巻 25, 番号 10. pp. 3997-4009.
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AU - Bergstrom, Donald A.

AU - Dacwag, Caroline S.

AU - Tapscott, Stephen J.

AU - Imbalzano, Anthony N.

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