N-linked carbohydrate on human leukocyte antigen-C and recognition by natural killer cell inhibitory receptors

Eishi Baba, Robert Erskine, Jonathan E. Boyson, George B. Cohen, Daniel M. Davis, Pratap Malik, Ofer Mandelboim, Hugh T. Reyburn, Jack L. Strominger

研究成果: ジャーナルへの寄稿記事

13 引用 (Scopus)

抜粋

The possible role of carbohydrate in the interaction of HLA-C with a human inhibitory natural Killer cell Immunoglobulin-like Receptor with two Ig domains, KIR2DL1, was investigated. Transfectants of 721.221 (a class I MHC-negative human B cell line) expressing only HLA-Cw4 or -Cw6 or their respective non-glycosylated mutants (N86Q, S88A) were made. The binding of a KIR2DL1-Ig fusion protein to the nonglycosylated mutant HLA-Cw4- or -Cw6-expressing cells was markedly decreased compared to the wild type-expressing cells. The ability to induce an inhibitory signal in the NK tumor line YTS transfected with KIR2DL1 was also impaired in the nonglycosylated mutant expressing cells. Furthermore, in a second functional assay, mutant HLA-Cw4 and -Cw6 molecules had impaired ability to induce signal transduction in BW cells expressing a KIR2DL1-CD3 zeta chain chimeric protein. Thus, the deletion of the N-linked glycosylation signal in HLA-Cw4 and -Cw6 greatly reduced recognition by KIR2DL1. Alternative interpretations of the data are discussed.

元の言語英語
ページ(範囲)1202-1218
ページ数17
ジャーナルHuman Immunology
61
発行部数12
DOI
出版物ステータス出版済み - 1 1 2000
外部発表Yes

    フィンガープリント

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

これを引用

Baba, E., Erskine, R., Boyson, J. E., Cohen, G. B., Davis, D. M., Malik, P., ... Strominger, J. L. (2000). N-linked carbohydrate on human leukocyte antigen-C and recognition by natural killer cell inhibitory receptors. Human Immunology, 61(12), 1202-1218. https://doi.org/10.1016/S0198-8859(00)00184-1