NAD(P)H oxidases in rat basilar arterial endothelial cells

Tetsuro Ago, Takanari Kitazono, Junya Kuroda, Yasuhiro Kumai, Masahiro Kamouchi, Hiroaki Ooboshi, Masanori Wakisaka, Tsukasa Kawahara, Kazuhito Rokutan, Setsuro Ibayashi, Mitsuo Iida

研究成果: ジャーナルへの寄稿記事

98 引用 (Scopus)

抄録

Background and Purpose - Reactive oxygen species (ROS) may play a critical role in the regulation of vascular tone and development of vascular diseases, such as stroke. NAD(P)H oxidase is a major source of ROS in vascular cells, including endothelial cells. It has been considered that Nox2 and Nox4 are exclusively expressed among Nox homologues in the endothelial cells of noncerebral blood vessels. However, the precise molecular identity of the NAD(P)H oxidase in the endothelial cells of the cerebral arteries is not fully understood. We examined the expression of Nox homologues and their activation mechanism in the endothelial cells of the cerebral arteries. Methods - We isolated and cultured basilar artery endothelial cells (BAECs) of Sprague-Dawley rats. Expression of NAD(P)H oxidase was examined by reverse-transcription- polymerase chain reaction (RT-PCR) and immunohistological staining. Results - RT-PCR disclosed abundant expression of Nox4 with marginal Nox2 in BAEC. In addition, Nox1 was expressed highly both at mRNA and protein levels in BAECs. Immunohistological staining also showed the prominent expression of Nox1 in the endothelial cells of the basilar artery. With respect to the cytosolic components of NAD(P)H oxidases, BAECs expressed p67phox and, to a lesser extent, p47phox, Noxo1, and Noxa1. Both NADH and NADPH induced superoxide production of the BAEC membranes. The phagocyte-type cytosolic components, p47phox and p67phox, significantly enhanced the NADH-induced superoxide production of the BAEC membranes, whereas the components failed to increase the NADPH-induced superoxide production. Conclusions - Nox1 is highly expressed in the endothelial cells of the cerebral arteries along with Nox2 and Nox4, and the endothelial NAD(P)H oxidase of the cerebral arteries may have a unique activation mechanism by the phagocyte-type cytosolic components.

元の言語英語
ページ(範囲)1040-1046
ページ数7
ジャーナルStroke
36
発行部数5
DOI
出版物ステータス出版済み - 5 1 2005

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NADPH Oxidase
Endothelial Cells
Basilar Artery
Cerebral Arteries
Superoxides
Blood Vessels
Phagocytes
NADP
NAD
Reverse Transcription
Reactive Oxygen Species
Cell Membrane
Staining and Labeling
Polymerase Chain Reaction
Vascular Diseases
Sprague Dawley Rats
Stroke

All Science Journal Classification (ASJC) codes

  • Clinical Neurology
  • Cardiology and Cardiovascular Medicine
  • Advanced and Specialised Nursing

これを引用

NAD(P)H oxidases in rat basilar arterial endothelial cells. / Ago, Tetsuro; Kitazono, Takanari; Kuroda, Junya; Kumai, Yasuhiro; Kamouchi, Masahiro; Ooboshi, Hiroaki; Wakisaka, Masanori; Kawahara, Tsukasa; Rokutan, Kazuhito; Ibayashi, Setsuro; Iida, Mitsuo.

:: Stroke, 巻 36, 番号 5, 01.05.2005, p. 1040-1046.

研究成果: ジャーナルへの寄稿記事

Ago, T, Kitazono, T, Kuroda, J, Kumai, Y, Kamouchi, M, Ooboshi, H, Wakisaka, M, Kawahara, T, Rokutan, K, Ibayashi, S & Iida, M 2005, 'NAD(P)H oxidases in rat basilar arterial endothelial cells', Stroke, 巻. 36, 番号 5, pp. 1040-1046. https://doi.org/10.1161/01.STR.0000163111.05825.0b
Ago, Tetsuro ; Kitazono, Takanari ; Kuroda, Junya ; Kumai, Yasuhiro ; Kamouchi, Masahiro ; Ooboshi, Hiroaki ; Wakisaka, Masanori ; Kawahara, Tsukasa ; Rokutan, Kazuhito ; Ibayashi, Setsuro ; Iida, Mitsuo. / NAD(P)H oxidases in rat basilar arterial endothelial cells. :: Stroke. 2005 ; 巻 36, 番号 5. pp. 1040-1046.
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abstract = "Background and Purpose - Reactive oxygen species (ROS) may play a critical role in the regulation of vascular tone and development of vascular diseases, such as stroke. NAD(P)H oxidase is a major source of ROS in vascular cells, including endothelial cells. It has been considered that Nox2 and Nox4 are exclusively expressed among Nox homologues in the endothelial cells of noncerebral blood vessels. However, the precise molecular identity of the NAD(P)H oxidase in the endothelial cells of the cerebral arteries is not fully understood. We examined the expression of Nox homologues and their activation mechanism in the endothelial cells of the cerebral arteries. Methods - We isolated and cultured basilar artery endothelial cells (BAECs) of Sprague-Dawley rats. Expression of NAD(P)H oxidase was examined by reverse-transcription- polymerase chain reaction (RT-PCR) and immunohistological staining. Results - RT-PCR disclosed abundant expression of Nox4 with marginal Nox2 in BAEC. In addition, Nox1 was expressed highly both at mRNA and protein levels in BAECs. Immunohistological staining also showed the prominent expression of Nox1 in the endothelial cells of the basilar artery. With respect to the cytosolic components of NAD(P)H oxidases, BAECs expressed p67phox and, to a lesser extent, p47phox, Noxo1, and Noxa1. Both NADH and NADPH induced superoxide production of the BAEC membranes. The phagocyte-type cytosolic components, p47phox and p67phox, significantly enhanced the NADH-induced superoxide production of the BAEC membranes, whereas the components failed to increase the NADPH-induced superoxide production. Conclusions - Nox1 is highly expressed in the endothelial cells of the cerebral arteries along with Nox2 and Nox4, and the endothelial NAD(P)H oxidase of the cerebral arteries may have a unique activation mechanism by the phagocyte-type cytosolic components.",
author = "Tetsuro Ago and Takanari Kitazono and Junya Kuroda and Yasuhiro Kumai and Masahiro Kamouchi and Hiroaki Ooboshi and Masanori Wakisaka and Tsukasa Kawahara and Kazuhito Rokutan and Setsuro Ibayashi and Mitsuo Iida",
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T1 - NAD(P)H oxidases in rat basilar arterial endothelial cells

AU - Ago, Tetsuro

AU - Kitazono, Takanari

AU - Kuroda, Junya

AU - Kumai, Yasuhiro

AU - Kamouchi, Masahiro

AU - Ooboshi, Hiroaki

AU - Wakisaka, Masanori

AU - Kawahara, Tsukasa

AU - Rokutan, Kazuhito

AU - Ibayashi, Setsuro

AU - Iida, Mitsuo

PY - 2005/5/1

Y1 - 2005/5/1

N2 - Background and Purpose - Reactive oxygen species (ROS) may play a critical role in the regulation of vascular tone and development of vascular diseases, such as stroke. NAD(P)H oxidase is a major source of ROS in vascular cells, including endothelial cells. It has been considered that Nox2 and Nox4 are exclusively expressed among Nox homologues in the endothelial cells of noncerebral blood vessels. However, the precise molecular identity of the NAD(P)H oxidase in the endothelial cells of the cerebral arteries is not fully understood. We examined the expression of Nox homologues and their activation mechanism in the endothelial cells of the cerebral arteries. Methods - We isolated and cultured basilar artery endothelial cells (BAECs) of Sprague-Dawley rats. Expression of NAD(P)H oxidase was examined by reverse-transcription- polymerase chain reaction (RT-PCR) and immunohistological staining. Results - RT-PCR disclosed abundant expression of Nox4 with marginal Nox2 in BAEC. In addition, Nox1 was expressed highly both at mRNA and protein levels in BAECs. Immunohistological staining also showed the prominent expression of Nox1 in the endothelial cells of the basilar artery. With respect to the cytosolic components of NAD(P)H oxidases, BAECs expressed p67phox and, to a lesser extent, p47phox, Noxo1, and Noxa1. Both NADH and NADPH induced superoxide production of the BAEC membranes. The phagocyte-type cytosolic components, p47phox and p67phox, significantly enhanced the NADH-induced superoxide production of the BAEC membranes, whereas the components failed to increase the NADPH-induced superoxide production. Conclusions - Nox1 is highly expressed in the endothelial cells of the cerebral arteries along with Nox2 and Nox4, and the endothelial NAD(P)H oxidase of the cerebral arteries may have a unique activation mechanism by the phagocyte-type cytosolic components.

AB - Background and Purpose - Reactive oxygen species (ROS) may play a critical role in the regulation of vascular tone and development of vascular diseases, such as stroke. NAD(P)H oxidase is a major source of ROS in vascular cells, including endothelial cells. It has been considered that Nox2 and Nox4 are exclusively expressed among Nox homologues in the endothelial cells of noncerebral blood vessels. However, the precise molecular identity of the NAD(P)H oxidase in the endothelial cells of the cerebral arteries is not fully understood. We examined the expression of Nox homologues and their activation mechanism in the endothelial cells of the cerebral arteries. Methods - We isolated and cultured basilar artery endothelial cells (BAECs) of Sprague-Dawley rats. Expression of NAD(P)H oxidase was examined by reverse-transcription- polymerase chain reaction (RT-PCR) and immunohistological staining. Results - RT-PCR disclosed abundant expression of Nox4 with marginal Nox2 in BAEC. In addition, Nox1 was expressed highly both at mRNA and protein levels in BAECs. Immunohistological staining also showed the prominent expression of Nox1 in the endothelial cells of the basilar artery. With respect to the cytosolic components of NAD(P)H oxidases, BAECs expressed p67phox and, to a lesser extent, p47phox, Noxo1, and Noxa1. Both NADH and NADPH induced superoxide production of the BAEC membranes. The phagocyte-type cytosolic components, p47phox and p67phox, significantly enhanced the NADH-induced superoxide production of the BAEC membranes, whereas the components failed to increase the NADPH-induced superoxide production. Conclusions - Nox1 is highly expressed in the endothelial cells of the cerebral arteries along with Nox2 and Nox4, and the endothelial NAD(P)H oxidase of the cerebral arteries may have a unique activation mechanism by the phagocyte-type cytosolic components.

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