NAD+-dependent conversion of 20-OH-LTB4 to 20-COOH-LTB4 by a cell-free system of human polymorphonuclear leukocytes

Hideki Sumimoto, Koichiro Takeshige, Shigeki Minakami

研究成果: Contribution to journalArticle

13 被引用数 (Scopus)

抄録

Human polymorphonuclear leukocytes, but not mononuclear leukocytes, platelets, or erythrocytes, oxidized 20-hydroxy-leukotriene B4 (20-OH-LTB4) to 20-carboxy-LTB4 (20-COOH-LTB4). 20-OH-LTB4 was quantitatively converted to 20-COOH-LTB4 by the sonicate of polymorphonuclear leukocytes in the presence of NAD+, with an optimal pH of about 7.9. NADP+ could not replace NAD+. The conversion was not inhibited by 2 mM pyrazole, a potent inhibitor of alcohol dehydrogenase. When LTB4 was incubated with the sonicate in the presence of NADPH and NAD+, 20-OH-LTB4 and 20-COOH-LTB4 appeared concomitantly with the disappearance of LTB4.

本文言語英語
ページ(範囲)864-870
ページ数7
ジャーナルBiochemical and Biophysical Research Communications
132
3
DOI
出版ステータス出版済み - 11 15 1985

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

フィンガープリント 「NAD<sup>+</sup>-dependent conversion of 20-OH-LTB<sub>4</sub> to 20-COOH-LTB<sub>4</sub> by a cell-free system of human polymorphonuclear leukocytes」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

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